FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

I-Fluorescein mono-beta-D-galactopyranoside, ekwabizwa ngokuba yi-FMG, yikhompawundi ye-fluorescent edla ngokusetyenziswa njenge-substrate kwiimvavanyo ezahlukeneyo ze-biochemical kunye ne-cell biology.Ithathwe kwi-methyl-beta-D-galactopyranoside ngokuyidibanisa ne-molecule ye-fluorescein.I-FMG isetyenziselwa ngokubanzi ukufunda umsebenzi we-beta-galactosidase, i-enzyme eyenza i-hydrolysis ye-lactose kwi-galactose kunye ne-glucose.Ngokusebenzisa i-FMG njenge-substrate, abaphandi banokujonga umsebenzi we-enzymatic we-beta-galactosidase ngomlinganiselo wokukhutshwa kwe-fluorescence.I-hydrolysis ye-FMG nge-beta-galactosidase ikhokelela ekukhululweni kwe-fluorescein, okubangelwa ukwanda kwesignali ye-fluorescent enokuthi ifakwe.I-FMG ingasetyenziswa njenge-probe ye-molecular ukufunda ubudlelwane obubophezelayo be-lectin (iiprotheni ezibophelela ngokukodwa kwi-carbohydrates) kwi-galactose equkethe i-carbohydrates.Ukubophelela kwe-FMG-lectin complexes kunokubonwa kwaye kulinganiswe ngokusekelwe kwiinguqu kwi-fluorescence emission.Kuko konke, i-FMG sisixhobo esisebenzayo ekufundeni umsebenzi we-enzyme kunye nokuqatshelwa kwe-carbohydrate, enika indlela efanelekileyo kunye neyobuthathaka yokulinganisa i-fluorescence kunye nokuvavanya ezi nkqubo zezinto eziphilayo.

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I-Fluorescein mono-beta-D-galactopyranoside (FMG) yimolekyuli esetyenziswa ngokuqhelekileyo kuphando lwebhayoloji njengenxalenye engaphantsi ukuze ibone ubukho kunye nomsebenzi we-beta-galactosidase enzyme.I-FMG iphuma kwi-lactose yeswekile kwaye idityaniswe ne-molecule ye-fluorescein.

Impembelelo ephambili ye-FMG kukuba i-hydrolyzed ngokukodwa nge-beta-galactosidase, i-enzyme ephula i-lactose kwi-galactose kunye ne-glucose.Le hydrolysis enzymatic ye-FMG ikhokelela ekukhululweni kwe-fluorescein, ekhupha uphawu oluqinileyo lwe-fluorescence.

Ukusetyenziswa okuphambili kwe-FMG kukufumanisa kunye nokulinganisa umsebenzi we-beta-galactosidase kwiisampuli ezahlukeneyo.Le enzyme ifumaneka kwizinto ezininzi eziphilayo, kubandakanywa ibhaktheriya kunye neeseli ze-mammalian, kwaye umsebenzi wayo unokubonisa iinkqubo ezahlukeneyo zeselula kunye neendlela ze-metabolic.

Ngokusebenzisa i-FMG njenge-substrate, umsebenzi we-beta-galactosidase unokulinganiswa ngokubeka iliso kwi-fluorescence ekhutshwe yi-fluorescein ekhululekile.Lo mlinganiso unokwenziwa kwiindidi zovavanyo lokuseta, kubandakanywa iimvavanyo ze-vitro kunye nezifundo zokucinga ngeeseli eziphilayo.

Ngapha koko, i-FMG ingasetyenziswa njengesixhobo sokufunda ukusasazwa kunye nokufakwa kwendawo ye-beta-galactosidase ngaphakathi kweeseli.Ngokusebenzisa ubuchule be-microscopy ye-fluorescent, abaphandi banokubona ngeso lengqondo i-fluorescence ekhutshwe yi-FMG phezu kwe-hydrolysis, ebavumela ukuba balandele umkhondo wesithuba kunye nomsebenzi wexeshana we-beta-galactosidase.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	C26H22O10
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	102286-67-9
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.