I-Fmoc-Asn(Trt)-OH CAS:132388-59-1

I-Fmoc-Asn(Trt)-OH ibambe indawo ephambili ekuhlanganisweni kwe-peptide, ngakumbi kwindibano elawulwayo yee-peptides ezine-asparagine. Iqela elikhuselayo le-Fmoc likhusela iqela le-amino, liqinisekisa iimpendulo ezikhethiweyo ngexesha lokudibanisa i-peptide, ngelixa iqela le-trityl libonelela ngomaleko owongezelelweyo wokhuseleko. Abaphandi basebenzisa kakhulu i-Fmoc-Asn(Trt)-OH ekuhlanganisweni kwee-peptides kwizicelo ezahlukeneyo, kubandakanya uphuhliso lwamayeza, izinto eziphilayo, kunye noyilo lwee-peptides ezisebenzayo. Ukuhambelana kwayo neendlela ezisemgangathweni zokwenziwa kwe-peptide, zombini kwizicwangciso zesigaba esiqinileyo kunye nesigaba sesisombululo, kuphucula ukusetyenziswa kwayo kwimizamo eyahlukeneyo yophando. Ukususwa okulawulwayo kwamaqela akhuselayo kuvumela ukufakwa kwe-asparagine kwizicwangciso ze-peptide, okufaka isandla kulwahluko lwee-peptides ezinokwenziwa. Ngaphezu koko, i-Fmoc-Asn(Trt)-OH ifumana uncedo ekuhlanganisweni kwee-peptides kuphando lwe-proteomics kunye nophando lweenkqubo zeseli. Ukusetyenziswa ngobuchule kweqela lokukhusela i-trityl kwenza ukuba i-asparagine reactivity isebenze ngokuchanekileyo, nto leyo enika abaphandi isixhobo esibalulekileyo sokuyila iipeptides ezinotshintsho oluthile. Ngamafutshane, i-Fmoc-Asn(Trt)-OH imi njengesixhobo esiguquguqukayo nesingenamsebenzi kwi-peptide chemistry, esinika abaphandi amandla okuphonononga nokuguqula i-asparagine reactivity kwiintlobo ngeentlobo zezicelo zesayensi.