I-HEPES CAS: 7365-45-9

I-HEPES idlala indima ebalulekileyo ekugcineni imeko-bume ye-pH ezinzileyo kwiinkqubo zenkcubeko yeseli, ukuqinisekisa ukuba iiseli ziyaphila kwaye zikhula ngokuthintela ukuguquguquka kwamanqanaba e-pH. Olu buchule bokugcina i-HEPES bubaluleke kakhulu ekugcineni iimeko ze-physiological zophando olusekelwe kwiseli kunye novavanyo lwamayeza, okukhokelela kwiziphumo ezithembekileyo neziphindaphindayo. Kwi-molecular biology kunye ne-biochemistry, i-HEPES isetyenziswa kakhulu kwiimvavanyo ze-enzyme ukulawula i-pH-sensitive enzyme reactions ngokufanelekileyo. Amandla ayo okugcina i-pH engaguqukiyo ngaphakathi koluhlu oluncinci (ngesiqhelo i-7.2-7.6) abalulekile ekuqinisekiseni ukuchaneka nokusebenza kakuhle kweenkqubo ze-biochemical, ezifana nokucocwa kweproteni, uhlalutyo lwe-nucleic acid, kunye nezifundo ze-metabolic. I-HEPES ikwasetyenziswa kwizicelo ze-electrophoresis, kubandakanya i-agarose gel electrophoresis kunye ne-polyacrylamide gel electrophoresis, apho inceda ekudaleni imeko-bume efanelekileyo yokwahlulwa kwe-DNA kunye neproteni. Iimpawu zayo zokugcina i-buffer zinegalelo kwiibhendi ezibukhali nezichazwe kakuhle, ziphucula isisombululo kunye nokuchaneka kweziphumo zovavanyo. Ngaphezu koko, i-HEPES isetyenziswa kakhulu kwishishini leyeza ekwenzeni iimveliso zebhayoloji, izitofu, kunye nee-reagents zokuxilonga. Ukuhambelana kwayo ne-biomolecules ezinobuthathaka kunye ne-cytotoxicity ephantsi kwenza ukuba ibe lukhetho olufanelekileyo lokugcina uzinzo kunye nokusebenza kwe-biopharmaceuticals ngexesha lokuveliswa kunye nokugcinwa. Ngokubanzi, i-HEPES isebenza njengearhente yokukhusela eguquguqukayo neyimfuneko enezicelo ezahlukeneyo kwi-biology yeseli, uphando lweemolekyuli, i-biochemistry, kunye nophuhliso lwamayeza, idlala indima ebalulekileyo ekuqinisekiseni impumelelo kunye nokuthembeka koluhlu olubanzi lweemvavanyo zebhayoloji kunye nezebhayoloji.