HEPES-Na CAS: 75277-39-3 Ixabiso lomvelisi

I-HEPES yetyuwa yesodium, ekwabizwa ngokuba yi-N-(2-Hydroxyethyl) piperazine-N'-2-ethanesulfonic acid sodium salt, sisithinteli se-zwitterionic esiqhele ukusetyenziswa kuphando lwebhayoloji kunye ne-biochemical.Umsebenzi wayo ophambili kukugcina inqanaba le-pH elizinzile kwiinkqubo ezahlukeneyo zokulinga.Ityuwa yesodium ye-HEPES inezinto ezininzi ezininzi, izinzile, kwaye ayinayo ityhefu, iyenza ilungele uluhlu olubanzi lwezicelo ezibandakanya inkcubeko yeseli, i-enzyme kinetics, i-immunoassays, kunye novavanyo lwebhayoloji yemolekyuli.Iyahambelana neesampulu zebhayoloji ezininzi kunye neenkqubo, ibonelela ngemekobume elawulwayo yolawulo lwe-pH oluchanekileyo.

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Buffering agent: HEPES ityuwa yesodium yesodium iqhele ukusetyenziswa njengesixhobo sokuthintela kuvavanyo lwebhayoloji nakwimichiza.Inceda ukugcina uluhlu lwe-pH oluzinzile, ngokukodwa kwi-physiological range (pH 7.2-7.6).Umthamo wayo we-buffering uwenza uxabiseke ekugcineni iimeko ezifanelekileyo zokwahluka kwe-enzymatic reaction, inkcubeko yeeseli, kunye nobuchule bebhayoloji yemolekyuli.

Ukukhuliswa kweeseli: I-HEPES yetyuwa yesodium isetyenziswa kakhulu njengesixhobo sokuthintela kwiiseli zenkcubeko.Ukukwazi ukugcina i-pH ezinzileyo kubalulekile ekukhuleni okufanelekileyo kunye nokusebenza kweeseli.I-HEPES isoloko ikhethwa ngaphezu kwezinye ii-agent ezikhuselayo kuba ingabonisi tshintsho lubalulekileyo kwi-pH xa ivezwe kwi-CO2 yomoya.

Izifundo ze-Enzyme: Ityuwa yesodium ye-HEPES iluncedo kakhulu kwizifundo ze-enzymatic apho imo engqongileyo ye-pH engaguqukiyo nelawulwayo ifuneka.Ikhusela ukuguquguquka okukhulu kwi-pH ngexesha lokuphendula kwe-enzymatic, iqinisekisa umsebenzi ofanelekileyo we-enzyme.

I-Electrophoresis: I-HEPES yetyuwa yesodium isetyenziswa ngokuqhelekileyo njenge-agent ephazamisayo kwiindlela ezahlukeneyo ze-electrophoretic ezifana ne-polyacrylamide gel electrophoresis (PAGE) kunye ne-agarose gel electrophoresis.Inceda ukugcina i-pH yesithinteli, esibalulekileyo ekwahluleni nasekuhlalutyeni i-nucleic acids kunye neeproteni.

Uvavanyo lwe-Biochemical: I-HEPES yetyuwa yesodium isoloko isetyenziswa kwiintlobo ezahlukeneyo zovavanyo lwe-biochemical, kubandakanywa uvavanyo lwe-enzyme, i-protein quantification assays, kunye ne-spectrophotometric assays.Inceda ukugcina uluhlu lwe-pH olufunwayo olufunekayo kwiziphumo ezichanekileyo kunye nokuveliswa kwakhona.

Ukwenziwa kweziyobisi: Ityuwa yesodium ye-HEPES ikwasetyenziswa ekuxutyweni kwamachiza njenge-agent ephazamisayo ukuzinzisa ukwakhiwa kwamachiza kunye nokugcina uluhlu lwe-pH olufunekayo.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	I-C8H19N2NaO4S
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	75277-39-3
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.