Iodoacetamide CAS:144-48-9 Ixabiso lomenzi

Iodoacetamide yikhemikhali esetyenziswa kakhulu kuphando lwe-biochemistry kunye ne-molecular biology. Yi-thiol-reactive compound eguqula ngokukodwa ii-cysteine residues kwiiproteni ngokwenza i-covalent bond. Olu tshintsho ludla ngokusetyenziswa kwiindlela zohlalutyo lweproteni ukuvimba ii-cysteine residues, njengakwizifundo zeproteomics apho isetyenziselwa ukulebhelisha ngokukhetha kunye nokuvimba ii-cysteine residues ngaphambi kokugaya iiproteni kunye nohlalutyo lwe-mass spectrometry. Iodoacetamide ikwasetyenziswa kwizifundo zesakhiwo seproteni kunye nokuhlanganiswa kwee-peptides ezineelebheli..

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukuguqulwa kweCysteine: Iodoacetamide isabela kunye namaqela e-thiol akhululekileyo kwi-cysteine residues, yenze i-covalent bond kunye ne-cysteine sulfur. Olu tshintsho lunceda kuhlalutyo lweprotheyini ngokuthintela ii-cysteine residues kunye nokuthintela ii-reactions ezingafunekiyo okanye i-oxidation.

Ukugaya iiProteni: I-Iodoacetamide isetyenziswa rhoqo kwimisebenzi yokugaya iiproteni yohlalutyo lwe-mass spectrometry. Isabela kunye neentsalela ze-cysteine ngaphambi kokugaya i-enzyme, ithintela ukwakheka kwe-disulfide bond engafunekiyo kwaye igcina iproteni ikwimeko yayo ephantsi.

Izifundo zeProteomics: I-Iodoacetamide isetyenziswa kwiindlela zokulebhelisha ezibandakanya i-proteomics esekelwe kwi-mass spectrometry. Ngokusebenzisa indlela esebenza ngayo kunye nee-cysteine residues, ivumela ukulebhelisha okukhethiweyo kunye nokuchongwa kweepeptides ezine-cysteine.

Izifundo zoLwakhiwo lweeProteni: Iodoacetamide isetyenziswa kwizifundo zolwakhiwo ukuguqula iintsalela ze-cysteine ngokweekhemikhali kunye nokufunda umsebenzi wazo kunye nokubandakanyeka kwazo ekusongeni, ekubopheni, nasekutshintsheni kwesimo seproteni. Inokuba luncedo nakwizilingo zokudibanisa ukuze kujongwe ukusebenzisana kweeproteni neeproteni.

Ukwenziwa kweePeptides ezineelebheli: Iodoacetamide ingasetyenziselwa ukwenziwa kweepeptides ezineelebheli ngokuguqula iintsalela ze-cysteine ngeekhompawundi ezineelebheli ezingabonakaliyo, ezifana ne-deuterium okanye i-carbon-13. Ezi peptides zineelebheli emva koko zisetyenziswa kwizicelo ezahlukeneyo zophando, kubandakanya ukulinganiswa kweproteni kunye nezifundo ze-dynamics.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C2H4INO
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	144-48-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.