I-Iodonitrotetrazolium chloride CAS:146-68-9 Ixabiso lomenzi

I-Iodonitrotetrazolium chloride (INT) isetyenziswa rhoqo ukufumanisa umsebenzi wee-enzymes ze-dehydrogenase kwiinkqubo zebhayoloji. Ezi enzymes zibandakanyeka kwi-metabolism yeseli kwaye zidlala indima ebalulekileyo kwiindlela ezahlukeneyo ze-biochemical. I-INT iluncedo ngakumbi kwiimvavanyo ezenzelwe ukuvavanya ukusebenza kweseli kunye nomsebenzi we-metabolism. Xa i-INT yongezwa kwisampulu yovavanyo, ukunciphisa idayi yi-enzymes ye-dehydrogenase kubangela ukwenziwa kwemveliso ebomvu ye-formazan. Ubunzulu bombala obomvu bunokulinganiswa nge-spectrophotometric, kubonelela ngomlinganiselo wobungakanani bomsebenzi we-enzyme kunye nokuphila kweseli. Oku kwenza i-INT ibe luncedo kwiindlela ezahlukeneyo zokusetyenziswa, kubandakanya uvavanyo lokwanda kweseli, uvavanyo lokuphila kwe-microbial, kunye novavanyo lwempembelelo yeziyobisi kwi-metabolism yeseli. Ukusetyenziswa kwayo kuphando kunye novavanyo lokuxilonga kunegalelo ekuqondeni ngcono iinkqubo ze-physiological kunye ne-pathological kwinqanaba leseli.