Iodonitrotetrazolium chloride CAS:146-68-9

Ukufumanisa umsebenzi we-dehydrogenase: I-INT ingasetyenziselwa ukufumanisa umsebenzi wee-enzymes ze-dehydrogenase kwiisampuli zebhayoloji. Ii-Dehydrogenase zii-enzymes ezibandakanyeka ekuphefumleni kweseli kunye neenkqubo ezahlukeneyo ze-metabolic. I-INT incitshiswa zii-enzymes ze-dehydrogenase, nto leyo ebangela ukwenziwa kwemveliso ebomvu ye-formazan. Ubunzulu bombala obomvu buhambelana ngqo nomsebenzi wee-enzymes ze-dehydrogenase ezikhoyo kwisampuli.

Uvavanyo lokuphila kweseli: I-INT ingasetyenziselwa ukuvavanya ukuphila kweseli kunye nomsebenzi we-metabolism. Ngokulinganisa ukwehla kwe-INT ukuya kwi-formazan, abaphandi banokumisela inani leeseli ezisebenzayo kwisampulu. I-INT encitshisiweyo yenza i-formazan kuphela kwiiseli ezisebenzayo ngokwe-metabolism, nto leyo eyenza ibe yidayi eluncedo yokuvavanya ukuphila kweseli kwizicelo ezifana novavanyo lwetyhefu yamayeza okanye uvavanyo lwenkcubeko yeseli.

Uphando lwe-microbiological: I-INT isetyenziswa rhoqo kwi-microbiology ukufumanisa nokubala iintsholongwane. Ingasetyenziselwa ukubonisa ukukhula kweebhaktheriya okanye iifungus ngokusekelwe kwimisebenzi yazo ye-metabolic. Ubukho bemveliso ye-formazan bubonisa ukukhula kweentsholongwane kwisampulu.

Ukuxilongwa kwezonyango: Uvavanyo olusekelwe kwi-INT lufumene ukusetyenziswa ekuxilongweni kwezonyango. Umzekelo, idayi ye-INT ingasetyenziselwa ukuvavanya umsebenzi wee-neutrophils (uhlobo lweseli emhlophe yegazi) ngokulinganisa amandla azo okunciphisa idayi. Ukungasebenzi kakuhle kwee-neutrophils kunxulunyaniswa nezifo ezahlukeneyo, kwaye uvavanyo lwe-INT lubonelela ngendlela ekhawulezayo nethembekileyo yokuvavanya umsebenzi wazo.