I-Nitro Blue Tetrazolium Chloride monohydrate CAS:298-96-4

Impendulo yeRedox: I-NBT chloride monohydrate idlula kwi-redox reaction xa incitshiswa zii-enzymes ezithile okanye ezinye ii-reducing agents. Le mpendulo iphumela ekudalweni kwe-dark blue formazan precipitate, ebonisa ubukho kunye nomsebenzi wezi enzymes ze-reductase.

Ukufunyanwa kwee-enzyme: I-NBT chloride monohydrate isetyenziswa kakhulu kwiimvavanyo ze-enzymes ukuze kufunyanwe umsebenzi wee-enzymes ezahlukeneyo ze-reductase. Umzekelo, isetyenziselwa ukufunyanwa kwee-dehydrogenases, i-succinate dehydrogenase, i-nitroreductases, kunye nezinye ii-enzymes ze-oxidoreductase. I-blue formazan precipitate eyenziweyo ibonisa ubukho kunye nomsebenzi wezi enzymes, nto leyo eyenza ukuba ibe luncedo ekuchazeni ii-enzymes kunye nezifundo zendawo.

Ukufaka idayi ye-Histochemical: I-NBT chloride monohydrate isetyenziswa kwiindlela zokufaka idayi ye-histochemical ukuze ibone ukusasazwa kunye nomsebenzi wee-enzymes ngaphakathi kwezicubu. Ingasetyenziselwa ukufunda indawo yee-enzymes kwiintlobo ezithile zeeseli okanye amacandelo ezicubu, okuvumela abaphandi ukuba baqonde ukubonakaliswa kwe-enzyme kunye nokusebenza ngaphakathi kweesampuli zebhayoloji.

Uvavanyo lokuPhila kweSeli: I-NBT chloride monohydrate isetyenziswa kuvavanyo lokuPhila kweSeli ukuvavanya umsebenzi we-metabolic kunye nemeko ye-redox yeeseli. Ukunciphisa i-NBT chloride kubonisa ubukho bee-enzymes ezisebenzayo ze-reductase kwiiseli, nto leyo ebonisa ukuba ziphilile kwaye ziyasebenza.

Izifundo ze-Microbiological: I-NBT chloride monohydrate isetyenziswa kwizifundo ze-microbiological ukufumanisa ubukho bee-enzymes ezithile ze-microbial. Le khompawundi ingasetyenziselwa ukuvavanya umsebenzi wee-enzymes ezithile eziveliswa yibhaktheriya okanye ifungi, inceda ekuchongeni nasekuchazeni iintlobo ze-microbial.