I-Nitrotetrazolium blue chloride CAS:298-83-9

I-Nitrotetrazolium blue chloride, ekwaziwa ngokuba yi-NBT okanye i-NBT-Cl, yikhemikhali esetyenziswa kakhulu kwiimvavanyo zebhayoloji kunye nezebhayoloji. Yipowder etyheli etyheli enyibilikayo emanzini.

I-NBT isetyenziswa rhoqo njengesalathisi sokufumanisa ubukho bee-superoxide radicals okanye ii-enzymes ezizivelisayo. Itshintsha umbala xa incitshiswa zii-superoxide radicals, itshintsha ukusuka kumthubi ukuya kwi-deep blue formazan precipitate. Le mpendulo idla ngokusetyenziswa kwiimvavanyo zokufunda uxinzelelo lwe-oxidative, ukuphefumla kweseli, kunye nomsebenzi wee-enzyme.

I-NBT isetyenziswa kakhulu kwiindlela ezahlukeneyo, ezinje ngokunciphisa i-nitroblue tetrazolium, ukuze kubonwe ukuveliswa kwe-superoxide kwiiseli kunye nezicubu. Ikwasetyenziswe kwi-immunohistochemistry kunye ne-histochemistry ukufumanisa nokuqonda ezinye ii-enzymes kunye nezinto zeseli.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-Nitrotetrazolium blue chloride (NBT) yidayi esetyenziswa kakhulu kwiimvavanyo zebhayoloji kunye nezebhayoloji. Isetyenziswa kakhulu njengesalathisi se-redox ukufumanisa nokubona ubukho bee-enzymes ze-dehydrogenase kwiisampuli zebhayoloji.

Xa i-NBT incitshiswa zii-enzymes ze-dehydrogenase, itshintsha umbala ukusuka kumbala otyheli okhanyayo ukuya kumbala oluhlaza okwesibhakabhaka omnyama we-formazan. Olu tshintsho luvumela abaphandi ukuba bachonge umsebenzi kunye nendawo yee-enzymes ze-dehydrogenase kwiithishu ezahlukeneyo kunye neeseli. Ngenxa yoko, i-NBT isetyenziswa rhoqo kwiimvavanyo zokuxilonga kunye nezifundo ze-immunohistochemical ukuvavanya umsebenzi we-metabolism yeseli kunye nokusebenza kwee-enzyme.

Ngokulinganisa ubukhulu bombala oluhlaza okwesibhakabhaka owenziweyo, abaphandi banokuhlalutya ngokwenani umsebenzi we-enzyme ye-dehydrogenase, ebalulekileyo ekuqondeni iinkqubo zeseli ezifana noxinzelelo lwe-oxidative, ukuphefumla kweseli, kunye ne-metabolism ye-carbohydrates kunye ne-lipids. Olu lwazi lubaluleke kakhulu kwiinkalo ezahlukeneyo, kubandakanya i-biochemistry, i-cell biology, kunye nokuxilongwa kweklinikhi.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C40H30ClN10O6+
Uvavanyo	99%
Inkangeleko	Umgubo otyheli
Inombolo ye-CAS	298-83-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.