I-Nitrotetrazolium Blue Chloride CAS: 298-83-9

I-Nitrotetrazolium Blue Chloride (NBT) sisalathisi se-redox esisetyenziswa ngokuqhelekileyo kwiimvavanyo zebhayoloji kunye ne-biochemical.Ngumgubo omthubi ophaphathekileyo ojika ube luhlaza okwesibhakabhaka xa uncitshisiwe, nto leyo eyenza kube luncedo ekuboneni ubukho bee-enzymes ezithile kunye nomsebenzi we-metabolic.

I-NBT iphendula kunye nabathwali be-electron kunye nee-enzyme ezifana ne-dehydrogenases, ezibandakanyeka kwiinkqubo ezahlukeneyo zeselula.Xa i-NBT incitshiswa ngezi enzyme, yenza i-formazan precipitate eluhlaza okwesibhakabhaka, evumela ukubonwa okubonakalayo okanye i-spectrophotometric.

Le reagent isetyenziswa ngokuqhelekileyo kwiimvavanyo ezifana novavanyo lokunciphisa i-NBT, apho isetyenziselwa ukuvavanya umsebenzi wokuphefumula wokuphefumula kwamaseli omzimba.Isenokusetyenziselwa ukufunda imisebenzi ye-enzyme kunye neendlela zemetabolism kuphando olunxulumene noxinzelelo lwe-oxidative, ukusebenza kweeseli, kunye nokwahlulahlula kweeseli.

I-NBT ifumene usetyenziso kwiinkalo ezahlukeneyo, kubandakanya i-microbiology, i-immunology, kunye nebhayoloji yeseli.Iyaguquguquka, izinzile, kwaye kulula ukuyisebenzisa, iyenza ibe lukhetho oludumileyo kwiiprothokholi ezininzi zovavanyo.

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Isicelo kunye nesiphumo

Isiphumo esiphambili se-NBT kukubunjwa kwe-formazan eluhlaza okwesibhakabhaka xa iyancipha ngama-enzyme athile.Olu tshintsho lombala luvumela ukubonwa okubonakalayo okanye i-spectrophotometric yomsebenzi we-enzyme.

I-NBT ineentlobo ngeentlobo zezicelo kuphando kunye noxilongo.Nazi ezinye zeendlela eziphambili zokusetyenziswa:

Uvavanyo lomsebenzi we-Enzyme: I-NBT ingasetyenziselwa ukulinganisa umsebenzi we-dehydrogenases, ebandakanyeka kwiinkqubo ezifana nokuphefumla kweselula kunye ne-metabolism.Ngokubeka iliso ekunciphiseni i-NBT kwi-formazan, abaphandi banokuhlola umsebenzi wezi enzymes.

Uvavanyo lwe-immune cell function: I-NBT isetyenziswa ngokuqhelekileyo kuvavanyo lokunciphisa i-NBT ukuvavanya umsebenzi wokuphefumula wokuphefumula kwamaseli omzimba, ngakumbi i-phagocytes.Uvavanyo lulinganisa ukukwazi kwezi seli ukuvelisa iintlobo ze-oksijini ezisebenzayo, ezinokunciphisa i-NBT kwaye zenze i-blue precipitate.

Uphando lweMicrobiology: I-NBT iqeshwe kwi-microbiology ukufunda i-microbial metabolism kunye nokuvavanya umsebenzi wee-enzymes ezithile.Ngokomzekelo, isetyenziselwe ukufumanisa i-nitrate ye-nitrate yebhaktiriya okanye i-formazan-forming bacteria.

Uphononongo lokusebenza kweeseli: Ukunciphisa i-NBT kuvumela abaphandi ukuba bahlole umsebenzi we-metabolic kunye nokusebenza kweeseli.Ngokulinganisa ubukhulu bemveliso ye-formazan eluhlaza okwesibhakabhaka, kunokwenzeka ukumisela inani leeseli ezisebenzayo kwisampulu enikiweyo.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	I-C40H30ClN10O6+
Isivavanyi	99%
Imbonakalo	Umgubo otyheli
Inombolo yeCAS.	298-83-9
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.