I-Proteinase K CAS:39450-01-6 Ixabiso lomenzi

I-Proteinase K yi-enzyme ye-serine protease esebenza kakhulu enokubangela i-hydrolysis yeebhondi ze-peptide kwiiproteni. Ivela kwi-albhamu ye-fungus i-Engyodontium kwaye yaziwa ngoluhlu lwayo olubanzi lwezinto ezithile ze-substrate. I-Proteinase K inokuqhawula ngempumelelo iibhondi ze-peptide kwicala le-C-terminal ye-hydrophobic, aliphatic, okanye i-aromatic amino acids, okwenza ukuba ibe luncedo kwiindlela ezahlukeneyo zebhayoloji ye-molecular kunye ne-biochemistry..

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukugaya nokucoca iiproteni: Iproteni K isetyenziswa rhoqo kwiinkqubo zokucoca iiproteni ukususa iiproteni okanye izinto ezingafunekiyo. Ingaqhawula ngempumelelo iibhondi zepeptide kwaye ityobe iiproteni, incedise ekuhlukaniseni nasekucoceni iiproteni ekujoliswe kuzo.

Ukutsalwa kweNucleic Acid: IProteinase K isetyenziswa rhoqo kwiiprotokholi zokukhupha iDNA kunye neRNA. Ngokuqhekeza iiproteni kunye nokususa iinuclease, inceda ekwahlukaniseni iDNA okanye iRNA esemgangathweni ophezulu ngaphandle kokuwohloka. IProteinase K inokusingatha iintlobo ngeentlobo zeesampuli, kubandakanya igazi, izicubu, okanye iiseli.

Ukucocwa kwe-PCR: I-Proteinase K ikwaluncedo kwiinkqubo zokucoca i-PCR. Ingasusa ii-enzymes ezingafunekiyo, ii-primers, ii-nucleotides, okanye ezinye izinto ezingcolileyo ezinokuphazamisana nokusetyenziswa kwazo ngezantsi. Ukucocwa kwe-Proteinase K kunceda ekufezekiseni iimveliso ze-PCR ezicocekileyo ezifanelekileyo uhlalutyo olongezelelweyo.

Ukuprinta iminwe ye-DNA: Kwi-DNA fingerprinting okanye kuhlalutyo lwe-forensic, iProteinase K isetyenziselwa ukukhupha i-DNA kwiisampuli ezahlukeneyo zebhayoloji, njengegazi, amathe, okanye iinwele. Amandla ayo okonakalisa iiproteni anceda ekuqhekezeni ii-membrane zeseli, ikhuphe i-DNA ukuze ihlalutywe kamva.

I-Histology kunye ne-Immunohistochemistry: I-Proteinase K inceda ekufumaneni i-antigen kwiindlela ze-histology kunye ne-immunohistochemistry. Ingaqhekeza amakhonkco anqamlezileyo enziwe ngexesha lokufakelwa kwesampuli, ivumela ukufikelela ngcono kwi-antigens kunye nokuphucula umgangatho wokufaka imibala kwiindawo zezicubu.

Ukungasebenzi kwe-Enzyme: I-Proteinase K idla ngokusetyenziselwa ukuvala ii-enzymes. Amandla ayo okutshabalalisa ii-enzymes ngokufanelekileyo athintela ukungcola okuvela kwiimpendulo zangaphambili, nto leyo eyenza ukuba ibe sisixhobo esibalulekileyo kwiimvavanyo ezininzi zebhayoloji yemolekyuli.

Izifundo zoBume beProtein kunye neMisebenzi: IProteinase K isetyenziswa kuphando lokufunda ulwakhiwo lweprotein kunye nomsebenzi wayo. Ngokuqhawula ngokukhetha iibhondi zepeptide kwiindawo ezithile, inokunceda ekuchongeni iindawo ezibalulekileyo okanye iindawo ezisebenzayo kwiiprotein..

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C29H27N2O12P
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	39450-01-6
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.