I-PUROMYCIN DIHYDROCHLORIDE CAS:58-58-2

Impembelelo kwiProtein Synthesis: I-Puromycin ifana ne-3'-end ye-aminoacyl-tRNAs kwaye ingangena kwi-A-site ye-ribosomes ngexesha lokuguqulelwa. Nje ukuba ifakwe, ibangela ukupheliswa kwangaphambili kokwenziwa kweproteni, okubangela ukukhululwa kweepolypeptides ezinqunqiweyo. Oku kwenza i-puromycin ibe sisixhobo esiluncedo sokufunda ukwenziwa kweproteni kunye namazinga okujika kwiiseli.

Uphawu Lokukhetha: I-Puromycin idla ngokusetyenziswa njengophawu lokukhetha kwiimvavanyo zobunjineli bemfuza. Iiseli eziye zatshintshwa ngempumelelo okanye zatshintshwa ngejini efunekayo okanye ukuguqulwa kwemfuza zenziwa zikwazi ukumelana ne-puromycin ngokufaka ijini yokumelana ne-puromycin. Ukongezwa kwe-puromycin kwinkcubeko yeseli kukhetha kuphela iiseli ezibonisa ijini yokumelana, okuvumela ukwahlulwa kunye nokwanda kweeseli eziguqulwe ngokwemfuza.

Uhlalutyo lokusebenzisana kweeProtein neeProtein: I-Puromycin ingadityaniswa nee-affinity tag ezithile okanye ii-antibodies ukuze kufundwe ukusebenzisana kweeprotein neeprotein. Ngokudibanisa iiprotein ezisebenzisanayo ne-puromycin, kunokwenzeka ukuzinzisa nokwahlula ezi protein complexes ukuze kuhlalutywe ngakumbi.

Uphononongo lokuphindaphindeka kweentsholongwane: I-Puromycin ingasetyenziselwa ukufunda ukuphindaphindeka kweentsholongwane kwinkcubeko yeseli. Ngokufaka i-puromycin kwiiseli ezineentsholongwane, ukwenziwa kweproteni entsha yentsholongwane kunokumiswa, okuvumela abaphandi ukuba baphande indima yeeproteni ezithile zentsholongwane kumjikelo wokuphindaphindeka..