I-Tapso Sodium CAS: 105140-25-8 Ixabiso lomvelisi

Uzinziso lwe-pH: I-TAPS isoloko isetyenziswa njengesithinteli ukuzinzisa i-pH yezisombululo kwimifuniselo eyahlukeneyo kunye nokusetyenziswa.Ine-pKa ekufutshane ne-pH ye-physiological, malunga ne-8.5, iyenza ukuba ifaneleke ngokukodwa ukugcina i-pH ezinzileyo kwiinkqubo zebhayoloji.

Izifundo zeprotheyini: I-TAPS isetyenziswa kakhulu kwiprotein biochemistry kunye ne-structural biology studies.Idla ngokusetyenziswa njenge agent ephazamisayo kwizisombululo zeprotheyini kunye nezithinteli ukugcina ukuhambelana kweprotein yendalo kunye nozinzo ngexesha lokucocwa, ukugcinwa, kunye novavanyo.I-TAPS iluncedo ngakumbi xa isebenza ngeeproteni ezibuthathaka kutshintsho lwe-pH.

Uvavanyo lwe-enzyme: I-TAPS isetyenziswa rhoqo njengesithinteli kwiimvavanyo ze-enzymatic, apho i-pH kufuneka ilawulwe ngokuchanekileyo.Umthamo wayo okhethekileyo wokubhufa kunye nokuzinza kuyenza ibe lolona khetho lukhethwayo kuluhlu olubanzi lweentshukumo ze-enzymatic.

Ukukhuliswa kweeseli: I-TAPS ihlala ibandakanyiwe kumajelo eendaba enkcubeko yeseli njenge-agent ekhuselayo ukugcina ezona meko zilungileyo ze-pH zokukhula kweeseli.I-biocompatibility yayo kunye nokuzinza kwenza kube luncedo kwiindidi ezahlukeneyo zeeseli kuzo zombini uphando kunye nokusetyenziswa kwezoshishino.

I-Electrophoresis: I-TAPS ingasetyenziswa njenge-agent ephazamisayo kwiindlela ze-gel electrophoresis, ezifana ne-SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis).Inceda ukugcina i-pH ezinzileyo ngexesha lokwahlula kunye nohlalutyo lweeprotheni, i-nucleic acids, kunye nezinye i-biomolecules.