TOPS CAS:40567-80-4 Ixabiso lomvelisi

I-sodium 3-(N-ethyl-3-methylanilino) propanesulfonate, eyaziwa ngokuba yi-MESNa, inefuthe elithile kunye nesicelo kwi-biochemistry kunye ne-biology ye-molecular.Isetyenziswe ngokuyinhloko njenge-arhente yokunciphisa ukukhusela amaqela e-thiol (-SH) kwiiprotheni kunye neepeptides.

I-MESNa isebenza njenge-arhente yokunciphisa enamandla, ekwazi ukuguqula iibhondi ze-disulfide (-SS-) kwiiprotheni zibe ngamaqela e-sulfhydryl (-SH).Le nkqubo yokunciphisa ibalulekile kwizicelo ezahlukeneyo zesayensi, kubandakanywa uhlalutyo lweprotheyini, ukuhlanjululwa, kunye nobunjineli beprotheni.

Ezinye izicelo ezithile ze-MESNa ziquka:

I-Protein Denaturation: I-MESNa ingasetyenziselwa ukudibanisa iiprotheni ngokuphula iibhondi ze-disulfide, ngaloo ndlela ityhila isakhiwo seprotheni kunye nokuququzelela uhlalutyo olongezelelweyo okanye ukuhlanjululwa kweprotheni.

Ukunciphisa i-Protein Aggregation: I-MESNa inokuthintela ukuhlanganiswa kweeprotheyini ngokunciphisa iibhondi ze-disulfide, ukuqinisekisa ukuzinza kweprotheni kunye ne-solubility kwisisombululo.

I-Protein Labeling: I-MESNa ingasetyenziselwa ukwazisa iileyibhile okanye ukuguqulwa kwiindawo ezithile ze-cysteine ​​​​kwiiprotheni ngokuphula iibhondi ze-disulfide kunye nokutyhila amaqela e-sulfhydryl (-SH).

I-Protein Refolding: I-MESNa isetyenziselwa ukuncedisa i-protein refolding emva kwe-denaturation ngokunciphisa iibhondi ze-disulfide kunye nokuncedisa ukubunjwa okuchanekileyo kwezakhiwo zeprotheni.