Tricine CAS:5704-04-1 Ixabiso lomvelisi

I-Tricine yi-zwitterionic organic compound kunye ne-chemical formula C6H13NO5S.Isetyenziswa ngokubanzi njenge-ejenti ye-buffering, ngokuyintloko kwi-biochemical and biological applications.Uphawu olwahlulayo lwe-Tricine sisixhobo sayo esikhethekileyo sokubhufa kuluhlu lwe-pH eneasidi kancinane, iyenza ibe luncedo ngakumbi kwimifuniselo efuna imo engqongileyo ye-pH ezinzileyo nechanekileyo.Iqhele ukusetyenziswa kwiprotein electrophoresis, ubuchule bebhayoloji yemolekyuli, uvavanyo lwe-enzymatic, kunye nemidiya yenkcubeko yeeseli.I-Tricine inceda ukugcina iimeko ezifanelekileyo kwiinkqubo ezahlukeneyo zebhayoloji, ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo kuphando kunye nohlalutyo.

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Kwi-biochemistry kunye ne-biology ye-molecular, "i-tricine effect" ibhekisela kwikhono le-tricine lokuphucula ukuhlukana kunye nesisombululo seeprotheni kwi-SDS-PAGE gels xa kuthelekiswa neenkqubo zendabuko ze-glycine.I-Tricine yi-amino acid encinci kune-glycine kwaye inokungena kwi-polyacrylamide gel matrix ngokulula, okukhokelela ekuhlukaneni kweprotheyini engcono.

Inkqubo ye-tricine buffer iluncedo ngakumbi ekwahluleni iiproteni ezinobunzima bemolekyuli (ngaphantsi kwama-20 kDa) kunye nokusombulula amabhanti afudukayo.Iqhele ukusetyenziswa kwi-Western blotting, ukuhlanjululwa kweprotheyini, kunye nezifundo zokubonisa iprotheni.I-Tricine iphinda isetyenziswe ngokudityaniswa nezinye ii-agent ezikhuselayo, ezifana ne-Bis-Tris okanye i-MOPS, ukunyusa uluhlu lwe-pH kunye nokuphucula isisombululo seprotheyini kwizicelo ezithile.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	C6H13NO5
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	5704-04-1
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.