X-GAL CAS:7240-90-6 Ixabiso lomvelisi

Ukutshintsha koMbala: I-X-Gal ayinamibala kodwa, xa i-hydrolysis yi-β-galactosidase, ijika ibe luhlaza.Olu tshintsho lombala luvumela ukubonwa okubonakalayo kunye nobungakanani bomsebenzi we-β-galactosidase.

Ukufunyanwa kweGene ye-LacZ: I-X-Gal isetyenziselwa ukuchonga iiseli okanye ulwakhiwo lwemfuza oluvakalisa ijini ye-lacZ.I-LacZ iqhele ukusetyenziswa njengengxelo yofuzo kwibhayoloji yemolekyuli ukuvavanya ukubonakaliswa kofuzo okanye umsebenzi wokukhuthaza ukufunda.

I-Colony Screening: I-X-Gal isetyenziswa rhoqo kwiimvavanyo zokuhlola ikholoni yebhaktheriya.I-LacZ-ebonisa i-bacterial colonies ibonakala iluhlaza okwesibhakabhaka xa ikhuliswe kwi-agar equlethe i-X-Gal, ivumela uchongo olulula kunye nokukhethwa kweekholoni ezine-lacZ-positive.

Uhlalutyo lwe-Gene Fusion: I-X-Gal iphinda isetyenziswe kwimifuniselo yokuhlanganiswa kwemfuza.Xa i-gene ekujoliswe kuyo idibaniswe ne-lacZ gene, i-X-Gal staining ingabonisa ipateni yokubonakalisa iprotheni yokudibanisa ngaphakathi kweseli okanye izicubu.

I-Protein Localization: I-X-Gal staining ingasetyenziselwa ukuphanda i-subcellular protein localization.Ngokudibanisa iprotheyini enomdla kwi-lacZ gene, umsebenzi we-β-galactosidase unokubonisa apho iprotheni ihlala khona ngaphakathi kwiseli.

I-X-Gal Analogues: Iifom ezilungisiweyo ze-X-Gal, ezifana ne-Bluo-Gal okanye i-Red-Gal, ziye zaphuhliswa ukuvumela ezinye iindlela zokuphuhlisa umbala.Ezi analogu zenza ukuba kubekho umahluko phakathi kweeseli ze-lacZ-positive kunye ne-lacZ-negative okanye izicubu ezisebenzisa imibala eyahlukileyo.