I-4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0

I-Substrate ye-enzymatic assays: I-4-Nitrophenyl-alpha-D-glucopyranoside isetyenziswa ngokuqhelekileyo njenge-substrate kwi-enzymatic assays ukulinganisa umsebenzi we-glycosidases kunye nezinye i-enzymes ezibandakanyekayo kwi-carbohydrate metabolism.Ezi enzyme zinokuqhawula ibhondi phakathi kwe-glucose kunye neqela le-4-nitrophenyl, ukukhulula imveliso ephuzi ebizwa ngokuba yi-4-nitrophenol.Isixa se-4-nitrophenol esiveliswayo sihambelana ngokuthe ngqo nomsebenzi we-enzyme, okuvumela ukulinganisa ubungakanani bomsebenzi we-enzyme.

Ukufumanisa umsebenzi we-enzyme: Ngokusebenzisa i-4-Nitrophenyl-alpha-D-glucopyranoside njenge-substrate, umsebenzi we-enzyme ethile unokulinganiswa.Ngokomzekelo, i-enzymes ye-glycosidase efana ne-beta-glucosidase, i-alpha-glucosidase, okanye i-beta-galactosidase inokuqhawula i-compound, ikhuphe i-4-nitrophenol, enokuthi ibonwe i-photometrically.Le ndlela isetyenziswa ngokubanzi kwiinkqubo ezahlukeneyo zonyango, amayeza, kunye ne-biotechnological.

Ukuhlolwa komgangatho ophezulu: Ngenxa yokuhambelana kwayo kunye neenkqubo zokufumanisa ngokuzenzekelayo, i-4-Nitrophenyl-alpha-D-glucopyranoside isetyenziswa ngokuqhelekileyo kwiimvavanyo eziphezulu zokuvavanya.Le khompawundi ivumela ukubonakaliswa okukhawulezayo kunye nokusebenza kakuhle komsebenzi we-enzyme, okwenza kube luncedo ekufumaneni iziyobisi, ubunjineli be-enzyme, kunye nezifundo zokunqanda i-enzyme.

Izicelo zokuxilonga: I-4-Nitrophenyl-alpha-D-glucopyranoside ingasetyenziswa kwizicelo zokuxilonga ukufumanisa ubukho okanye umsebenzi wee-enzyme ezithile ezinxulumene nezifo ezahlukeneyo.Ngokomzekelo, umsebenzi we-enzyme ethile ingasetyenziselwa ukuxilongwa kwezifo ezithile, kwaye i-4-Nitrophenyl-alpha-D-glucopyranoside ingasetyenziselwa njenge-substrate yokulinganisa umsebenzi wale enzyme kwiisampuli zekliniki.