4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

I-enzyme substrates: I-4NPM ingasetyenziselwa njenge-substrate yee-enzymes ezahlukeneyo, kuquka i-glycosidases kunye ne-enzymes ehambelana nayo.Ezi enzymes ziqhekeza ibhondi ye-glycosidic phakathi kwe-mannose kunye ne-4NPM, okubangela ukukhululwa kwe-nitrophenyl moiety.Ubungakanani be-substrate hydrolysis bunokulinganiswa nge-spectrophotometrically ngokubeka esweni ukufunxa kweqela le-nitrophenyl elikhutshiweyo kumgama wobude obuthile.Oku kuvumela abaphandi ukuba bahlole umsebenzi we-enzyme kunye ne-kinetics.

Uvavanyo lwe-carbohydrate metabolism: Ngokusebenzisa i-4NPM njenge-substrate, abaphandi banokufunda umsebenzi we-enzymes echaphazelekayo kwi-carbohydrate metabolism, njenge-alpha-mannosidases.Ezi enzymes ze-hydrolyze iibhondi ze-glycosidic kwi-mannose-containing compounds, kwaye umsebenzi wabo unokulinganiswa ngokubeka esweni ukukhululwa kwe-nitrophenyl moiety kwi-4NPM.

Izifundo ze-Glycosylation: I-4NPM ingasetyenziselwa kwiimvavanyo zokuphanda i-enzymes ezibandakanyekayo kwiinkqubo ze-glycosylation.I-Glycosylation yinkqubo yokudibanisa iimolekyuli zeswekile kwiiprotheni okanye ezinye iimolekyuli, kwaye ezininzi ii-enzymes zibandakanyeka kule nkqubo.Ngokusebenzisa i-4NPM njenge-substrate eyamkelekileyo, abaphandi banokulinganisa ukuhanjiswa kwe-sugar moiety kwi-4NPM ngama-enzyme athile abandakanyekayo kwi-glycosylation reactions.

Ukuhlolwa kwe-enzyme inhibitors okanye i-activators: I-4NPM ingasetyenziselwa kwiimvavanyo eziphezulu zokuvavanya ukuchonga iikhompawundi ezivimbela okanye zisebenze i-enzymes ethile.Ngokuphonononga umphumo weemvavanyo zokuvavanya kwi-hydrolysis okanye ukuguqulwa kwe-4NPM yi-enzymes ekujoliswe kuyo, abaphandi banokuchonga ii-agent ezinokuthi zonyango okanye iiprobes zekhemikhali eziluncedo zokufunda umsebenzi we-enzyme.