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I-PNPG CAS: 3150-24-1 Ixabiso lomvelisi
I-PNPG, okanye i-p-nitrophenyl β-D-glucopyranoside, i-substrate encinci ye-molecule ehlala isetyenziselwa kwiimvavanyo ze-biochemical ukulinganisa umsebenzi we-enzymes ye-glucosidase.Ayinambala kwaye ayinayo i-fluorescent, kodwa xa i-hydrolysis yi-glucosidase, iguqulwa ibe yi-p-nitrophenol, enombala otyheli kwaye ibonakala ngokulula kwi-spectrophotometrically.
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ONPG CAS: 369-07-3 Ixabiso lomvelisi
I-O-nitrophenyl-β-D-galactopyranoside (ONPG) i-substrate yokwenziwa esetyenziswa kwi-biochemical and molecular biology assays ukulinganisa umsebenzi we-enzyme β-galactosidase.Isetyenziswa ngokuqhelekileyo kwiimvavanyo zokubona ukubonakaliswa kofuzo kwiinkqubo zebhaktheriya, ezifana ne-Escherichia coli.ONPG yikhompawundi engenambala ecatshulwe yi-β-galactosidase, ekhokelela ekukhutshweni kwekhompawundi emthubi, i-o-nitrophenol.Umbala otyheli oveliswayo unokulinganiswa nge-spectrophotometrically, ukubonelela ngomlinganiselo ongathanga ngqo womsebenzi we-enzyme.Uvavanyo olusebenzisa i-ONPG luhlala lubizwa ngokuba yi-ONPG assay kwaye yindlela esetyenziswa ngokubanzi kuphando lwebhayoloji ye-molecular ukuvavanya amanqanaba okubonakaliswa kofuzo olulawulwa yi-lac. i-operon kwiiseli zebhaktheriya.
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I-Nitrotetrazolium Blue Chloride CAS: 298-83-9
I-Nitrotetrazolium Blue Chloride (NBT) sisalathisi se-redox esisetyenziswa ngokuqhelekileyo kwiimvavanyo zebhayoloji kunye ne-biochemical.Ngumgubo omthubi ophaphathekileyo ojika ube luhlaza okwesibhakabhaka xa uncitshisiwe, nto leyo eyenza kube luncedo ekuboneni ubukho bee-enzymes ezithile kunye nomsebenzi we-metabolic.
I-NBT iphendula kunye nabathwali be-electron kunye nee-enzyme ezifana ne-dehydrogenases, ezibandakanyeka kwiinkqubo ezahlukeneyo zeselula.Xa i-NBT incitshiswa ngezi enzyme, yenza i-formazan precipitate eluhlaza okwesibhakabhaka, evumela ukubonwa okubonakalayo okanye i-spectrophotometric.
Le reagent isetyenziswa ngokuqhelekileyo kwiimvavanyo ezifana novavanyo lokunciphisa i-NBT, apho isetyenziselwa ukuvavanya umsebenzi wokuphefumula wokuphefumula kwamaseli omzimba.Isenokusetyenziselwa ukufunda imisebenzi ye-enzyme kunye neendlela zemetabolism kuphando olunxulumene noxinzelelo lwe-oxidative, ukusebenza kweeseli, kunye nokwahlulahlula kweeseli.
I-NBT ifumene usetyenziso kwiinkalo ezahlukeneyo, kubandakanya i-microbiology, i-immunology, kunye nebhayoloji yeseli.Iyaguquguquka, izinzile, kwaye kulula ukuyisebenzisa, iyenza ibe lukhetho oludumileyo kwiiprothokholi ezininzi zovavanyo.
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Neocuproine CAS: 484-11-7 Ixabiso lomvelisi
I-Neocuproine yi-agent ye-chelating esetyenziswa ngokuqhelekileyo kwizicelo ezahlukeneyo, kubandakanywa i-chemistry yohlalutyo kunye namayeza.Inobudlelwane obuqinileyo be-ion zobhedu kwaye yenza ii-complexes ezizinzile kunye nabo.Le propati yenza i-neocuproine ibe luncedo ekubhaqeni kunye nokulinganisa ubhedu kwizisombululo okanye iisampuli.Ukongeza, i-neocuproine ifundwe ngezicelo zayo ezinokubakho zonyango, ngakumbi ekunyangeni umhlaza kunye nezifo ze-neurodeergenerative.
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IPTG CAS: 367-93-1 Ixabiso lomvelisi
I-Isopropyl β-D-1-thiogalactopyranoside (IPTG) yi-analog synthetic ye-lactose esetyenziswa ngokuqhelekileyo kuphando lwebhayoloji ye-molekyuli kunye nezicelo ze-biotechnology.I-IPTG isetyenziselwa ukukhuthaza ukubonakaliswa kwejene kwiinkqubo zebhaktheriya, apho isebenza njenge-molecular trigger ukuqalisa ukukhutshelwa kweejene ezijoliswe kuzo.
Xa ifakwe kwi-medium yokukhula, i-IPTG ithathwa yibhaktheriya kwaye inokubophelela kwiprotheni ye-lac repressor, ukuyikhusela ekuvimbeleni umsebenzi we-lac operon.I-lac operon yiqela lezakhi zofuzo ezibandakanyekayo kwi-lactose metabolism, kwaye xa iprotheni ye-repressor isusiwe, iijini zibonakaliswa.
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I-HATU CAS: 148893-10-1 Ixabiso lomvelisi
I-HATU (1- [bis (dimethylamino) methylene] -1H-1,2,3-triazolo [4,5-b] pyridinium 3-oxid hexafluorophosphate) isetyenziswa ngokuqhelekileyo i-reagent yokudibanisa kwi-peptide synthesis kunye ne-organic chemistry.
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I-D-fucose CAS: 3615-37-0 Ixabiso lomvelisi
I-D-fucose yi-monosaccharide, ngokukodwa iswekile yekhabhoni emithandathu, eyeqela leeswekile ezilula ezibizwa ngokuba yi-hexoses.Yi-isomer ye-glucose, eyahlukileyo kwi-configuration yeqela elinye le-hydroxyl.
I-D-fucose ifumaneka ngokwendalo kwizinto ezahlukeneyo, kubandakanya iibhaktheriya, umngundo, izityalo kunye nezilwanyana.Idlala indima ebalulekileyo kwiinkqubo ezininzi zebhayoloji, ezifana nokubonakaliswa kweeseli, ukunamathela kweeseli, kunye ne-glycoprotein synthesis.Iyingxenye ye-glycolipids, i-glycoproteins, kunye ne-proteoglycans, ebandakanyekayo kwi-cell-to-cell communication kunye nokuqaphela.
Kubantu, i-D-fucose iphinda ibandakanyeke kwi-biosynthesis yezakhiwo ezibalulekileyo ze-glycan, ezifana ne-Lewis antigens kunye ne-antigens yeqela legazi, ezineempembelelo ekusebenzisaneni kwegazi kunye nokuchaphazeleka kwezifo.
I-D-fucose inokufumaneka kwimithombo eyahlukeneyo, kubandakanywa ukhula lwaselwandle, izityalo, kunye nokuvutshelwa kwe-microbial.Isetyenziselwa uphando kunye nezicelo ze-biomedical, kunye nokuveliswa kwamachiza athile kunye neekhompawundi zonyango.
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I-DDT CAS: 3483-12-3 Ixabiso lomvelisi
I-DL-Dithiothreitol, eyaziwa ngokuba yi-DTT, i-arhente yokunciphisa esetyenziswa ngokuqhelekileyo kuphando lwebhayoloji kunye ne-molecular biology.I-molecule encinci ene-thiol (equlethe isulfure) iqela kwinqanaba ngalinye.
I-DTT isetyenziswa rhoqo ukuphula iibhondi ze-disulfide kwiiprotheyini, ezinceda ukutyhila okanye ukudinwa.Oku kuncitshiswa kwamabhondi e-disulfide kubalulekile kwiinkqubo ezahlukeneyo zelabhoratri ezifana nokuhlanjululwa kweprotheyini, i-gel electrophoresis, kunye nezifundo zesakhiwo seprotheni.I-DTT ingasetyenziselwa ukukhusela amaqela e-thiol kunye nokuthintela i-oxidation ngexesha leenkqubo zovavanyo.
I-DTT yongezwa ngokuqhelekileyo kwizisombululo zokulinga kwiindawo ezincinci, kwaye umsebenzi wayo uxhomekeke kubukho be-oxygen.Kubalulekile ukuphatha i-DTT ngononophelo njengoko ivakalelwa emoyeni, ukushisa, kunye nokufuma, okunokunciphisa ukusebenza kwayo.
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D-(+)-Galactose CAS:59-23-4 Ixabiso lomvelisi
I-D-(+) -I-Galactose yishukela ye-monosaccharide kunye nenxalenye ebalulekileyo yeenkqubo ezininzi ze-biological.Yishukela eyenzeka ngokwemvelo efumaneka kukutya okuninzi, okufana neziqhamo, iimveliso zobisi, kunye nemifuno.
I-Galactose idla ngokuxutywa emzimbeni ngothotho lwee-enzymatic reaction.Idlala indima ebalulekileyo kunxibelelwano lweeseli, imveliso yamandla, kunye ne-biosynthesis yeemolekyuli ezibalulekileyo ezifana ne-glycolipids, i-glycoproteins, kunye ne-lactose.
Ngokwezicelo zayo, i-D-(+)-Galactose isetyenziswa ngokuqhelekileyo kwi-microbiology kunye ne-biotechnology njengomthombo wekhabhoni kwimidiya yenkcubeko yokukhula kwee-microorganisms ezahlukeneyo.Ikwasetyenziswa kwimveliso yeekhompawundi ezahlukeneyo ze-bioactive, amayeza, kunye neemveliso zokutya.Ukongezelela, isetyenziswa rhoqo njenge-arhente yokuxilongwa kwezonyango, ngakumbi kwiimvavanyo zokuvavanya umsebenzi wesibindi kunye nokufumanisa ukuphazamiseka kwemfuza okunxulumene ne-galactose metabolism.
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beta-D-Galactose pentaacetate CAS:4163-60-4
I-Beta-D-Galactose pentaacetate yikhemikhali ephuma kwi-galactose, i-sugar monosaccharide.Yenziwe nge-acetylating iqela ngalinye le-hydroxyl ye-molecule ye-galactose kunye namaqela amahlanu e-acetyl.
Le khompawundi isoloko isetyenziswa njenge-arhente ekhuselayo ye-galactose kwiimpendulo ezahlukeneyo zeekhemikhali kunye neenkqubo zokwenza.Ifom ye-pentaacetate inceda ukuzinzisa i-galactose kunye nokukhusela ukuphendula okungafunekiyo okanye ukuguqulwa ngexesha lokuphendula.
Ukongeza, le khompawundi inokusetyenziswa njengesandulela sokwenziwa kwezinye izinto eziphuma kwigalactose.Amaqela e-acetyl anokususwa ngokukhethiweyo ukuze afumane iintlobo ezahlukeneyo ze-galactose kunye namaqela athile asebenzayo.
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I-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide ityuwa yesodium CAS:129541-41-9
I-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide ityuwa ye-sodium yi-chemical compound esetyenziswa ngokuqhelekileyo kuphando lwebhubhoratri kunye nokuxilongwa.Ihlala ibizwa ngokuba yi-X-Gluc kwaye isetyenziswa ngokubanzi njenge-substrate yokufumanisa umsebenzi we-enzyme ye-beta-glucuronidase.
Xa i-beta-glucuronidase ikhona, iqhekeza ibhondi ye-glucuronide kwi-X-Gluc, okubangela ukukhululwa kwedayi eluhlaza ebizwa ngokuba yi-5-bromo-4-chloro-3-indolyl.Oku kusabela ngokuqhelekileyo kusetyenziselwa ukubona okanye ukubona i-spectrophotometrically ukubonakaliswa kwe-enzyme ye-beta-glucuronidase kwiiseli okanye kwizicubu.
Ifom yetyuwa ye-sodium ye-X-Gluc iphucula ukunyibilika kwayo kwizisombululo ezinamanzi, iququzelele ukusetyenziswa kwayo kwiimvavanyo zebhubhoratri.I-X-Gluc isetyenziswa ikakhulu kuphando lwebhayoloji yemolekyuli ukufunda ukuchazwa kofuzo, umsebenzi wokukhuthaza, kunye novavanyo lofuzo lwentatheli.Ingasetyenziselwa ukufumanisa ubukho bezinto eziphilayo ezivelisa i-beta-glucuronidase, ezifana neebhaktheriya ezithile, kwizifundo ze-microbiological.
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I-4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9
I-4-Nitrophenyl-beta-D-xylopyranoside i-substrate ye-chromogenic esetyenziswa kwiimvavanyo ze-enzymatic ukufumanisa nokulinganisa umsebenzi wee-enzyme ezibizwa ngokuba yi-beta-xylosidases.
