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I-2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2
I-2-Nitrophenyl-beta-D-glucopyranoside yi-chemical compound equkethe i-molecule ye-glucopyranoside ehlanganiswe neqela le-nitrophenyl.Ngokuqhelekileyo isetyenziswe njenge-substrate kwii-assays ze-enzymatic ukufumanisa kunye nokulinganisa umsebenzi wee-enzyme ezifana ne-beta-glucosidase.Iqela le-nitrophenyl linokucatshulwa yi-enzyme, okubangela ukukhululwa kwemveliso yombala ophuzi onokulinganiswa nge-spectrophotometrically.Le khompawundi iluncedo kakhulu ekufundeni i-enzyme kinetics kunye nokuhlolwa okuphezulu kwe-enzyme inhibitors okanye i-activators.Ikwasetyenziswa kuphando lwe-biochemical kuphando lwe-carbohydrate metabolism kunye ne-glycosidic-linkage-specific substrate.
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Tris Base CAS: 77-86-1 Ixabiso lomvelisi
I-Tris Base, eyaziwa ngokuba yi-Tromethamine okanye i-THAM, yikhompawundi ye-organic edla ngokusetyenziswa kwinkalo ye-biochemistry kunye ne-molecular biology.Ngumgubo omhlophe, oyikristale onyibilika kakhulu emanzini kwaye unevumba le-amine.I-Tris Base isoloko isetyenziswa njenge-agent ephazamisayo ukugcina i-pH ezinzileyo kwiimvavanyo ezahlukeneyo zebhayoloji kunye neenkqubo, ezifana ne-DNA kunye nezifundo zeprotheni.Inokusetyenziswa kwakhona ekuqulunqweni kwamachiza kunye nokuveliswa kwee-agent ezisebenza phezulu.Lilonke, i-Tris Base licandelo elibalulekileyo kwizicelo ezininzi zaselabhoratri apho ukugcinwa kwe-pH echanekileyo kubalulekile.
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I-4-Morpholineethanesulfonic acid CAS: 4432-31-9
I-4-Morpholineethanesulfonic acid, eyaziwa njenge-MES, yikhompawundi ye-zwitterionic esebenza njenge-buffering agent kuphando lwebhayoloji kunye ne-biochemical.Inceda ukugcina i-pH ezinzileyo malunga ne-6-7.5 kwaye isetyenziswa ngokubanzi ngenxa yetyhefu ephantsi kunye nokuhambelana neenkqubo ezahlukeneyo zebhayoloji kunye ne-enzymes.I-MES isetyenziswa kwi-electrophoresis, izifundo ze-enzyme, inkcubeko yeeseli, ukucocwa kweeprotheyini, kunye nezinye iinkqubo zovavanyo ezifuna ulawulo lwe-pH oluchanekileyo.
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I-4-Nitrophenyl-beta-D-glucopyranoside CAS: 2492-87-7
I-4-Nitrophenyl-beta-D-glucopyranoside i-substrate esetyenziswa ngokuqhelekileyo kwiimvavanyo ze-biochemical ukuhlola umsebenzi wee-enzymes ezifana ne-β-glucuronidase.Le ngqungquthela i-hydrolyzed yi-enzyme, ebangela ukukhululwa kwe-4-nitrophenol, enokulinganiswa ngokusebenzisa i-spectrophotometry.Ukusetyenziswa kwayo kwenza abaphandi bafunde iinkalo ezahlukeneyo zemetabolism yeziyobisi, i-toxicology, kunye noxilongo lweklinikhi olunxulumene nokuphendula kwe-glucuronidation.
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I-MOPSO yetyuwa yesodium CAS: 79803-73-9
Ityuwa ye-sodium ye-MOPSO yi-chemical compound ephuma kwi-MOPS (3- (N-morpholino) propanesulfonic acid).Yityuwa ye-zwitterionic buffer, okuthetha ukuba iqulethe zombini intlawulo elungileyo kunye nengalunganga, eyivumela ukuba igcine ngokufanelekileyo uzinzo lwe-pH kwimifuniselo eyahlukeneyo yebhayoloji kunye neyemichiza.
Ifom yetyuwa ye-sodium ye-MOPSO inikezela ngeenzuzo ezifana nokuphuculwa kwe-solubility kwizisombululo zamanzi, okwenza kube lula ukuphatha nokuzilungiselela.Idla ngokusetyenziswa njengesixhobo sokukhusela kwimidiya yenkcubeko yeeseli, ubuchule bebhayoloji yemolekyuli, uhlalutyo lweprotheyini, kunye nokuphendula kwe-enzyme.
Ityuwa ye-sodium ye-MOPSO inceda ukugcina i-pH ye-medium yokukhula kwinkcubeko yeeseli, inika indawo ezinzileyo yokukhula kweseli kunye nokusebenza.Kubuchule bebhayoloji yemolekyuli, izinzisa i-pH yemixube yokusabela kunye nokuqhuba izithinteli, iqinisekisa iziphumo ezichanekileyo nezithembekileyo kwi-DNA kunye ne-RNA yodwa, i-PCR, kunye ne-gel electrophoresis.
Ikwasetyenziswa kuhlalutyo lweprotheyini, isebenza njenge-agent ephazamisayo ngexesha lokucocwa kweprotheyini, ubungakanani kunye ne-electrophoresis.Ityuwa yesodium ye-MOPSO iqinisekisa iimeko ze-pH ezifanelekileyo zokuzinza kweprotheni kunye nomsebenzi kuzo zonke ezi nkqubo.
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I-4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4
I-4-Methylumbelliferyl-beta-D-glucopyranoside i-substrate esetyenziswa ngokuqhelekileyo kwiimvavanyo ze-enzymatic ukufunda umsebenzi we-beta-glucosidase enzymes.Xa isetyenzisiwe nge-beta-glucosidase, idlula i-hydrolysis, ibangele ukukhululwa kwe-4-methylumbelliferon, enokuthi ibonwe kwaye ilinganiswe ngokusebenzisa i-fluorescence spectroscopy.Le khompawundi isetyenziswa ngokubanzi kwiinkalo zebhayoloji, ibhayoloji yemolekyuli, kunye nebhayoloji yebhayoloji yovavanyo lomsebenzi we-enzyme kunye neenjongo zokuhlola.Ipropathi yayo ye-fluorescence yenza ukuba ibe novakalelo oluphezulu kwaye ifaneleke kwizicelo eziphezulu.
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METHYL Beta-D-GLUCOPYRANOSIDE HEMIHYDRATE CAS:7000-27-3
I-Methyl beta-D-glucopyranoside hemihydrate yikhompawundi yemichiza ekwiklasi yeglucopyranosides.Ngumgubo omhlophe wekristale onyibilikayo emanzini.Le khompawundi idla ngokusetyenziswa njengomthombo wecarbohydrate kumajelo eendaba enkcubeko yeseli kunye ne-substrate ye-enzymatic reactions kuphando lwe-biochemical kunye ne-biotechnological.Inokusebenza njengomzekelo wekhompawundi yokufunda i-carbohydrate metabolism, ukuthutha, kunye nokusetyenziswa kwiinkqubo ezahlukeneyo zebhayoloji.I-Methyl beta-D-glucopyranoside hemihydrate ifumana izicelo kwintsimi ye-glycobiology, i-enzymology, kunye nophuhliso lweziyobisi, apho isetyenziswe njengekhompawundi yesixhobo kwiimvavanyo ezahlukeneyo kunye nemifuniselo.
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I-AMPSO CAS: 68399-79-1 Ixabiso lomvelisi
I-AMPSO, okanye i-3 - [(1,1-dimethyl-2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, i-zwitterionic buffer edla ngokusetyenziswa kwi-biological and biochemical research.Inexabiso le-pKa elijikeleze i-7.9, eyenza ukuba ifaneleke ukugcina iimeko ezizinzileyo ze-pH kwiindawo ezahlukeneyo zovavanyo.I-AMPSO isoloko isetyenziswa kwimidiya yenkcubeko yeeseli, ukuhlanjululwa kweprotheyini, ukuhlolwa kwe-enzyme, i-electrophoresis gels, kunye nokulandelelana kwe-DNA.Inceda ukugcina uluhlu lwe-pH olufunwayo, ukuqinisekisa iimeko ezifanelekileyo zokukhula kweeseli, ukuzinza kweprotheni, umsebenzi we-enzyme, kunye nokwahlula ngokuchanekileyo kunye nohlalutyo lwe-biomolecules.Ngokukwazi ukumelana nokutshintsha kwe-pH okubangelwa ukongezwa kwe-acids okanye iziseko, i-AMPSO iyisixhobo esibalulekileyo ukugcina ulawulo lwe-pH oluchanekileyo kuluhlu lwemifuniselo yebhayoloji neyemichiza.
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I-Bis-tris hydrochloride CAS: 124763-51-5
I-Bis-tris hydrochloride yikhompawundi eneempawu zokuthintela ezidla ngokusetyenziswa kwimifuniselo yebhayoloji neyebhayoloji.Inceda ukugcina i-pH ezinzileyo kwaye isetyenziswe kwiprotheyini ye-electrophoresis, i-enzyme assays, inkcubeko yeseli, kunye nokuqulunqwa kwamayeza.Umsebenzi wayo oyintloko kukuxhathisa utshintsho kwi-pH xa i-acids okanye iziseko zongezwa kwisisombululo, okwenza kube sisixhobo esibalulekileyo kwizicelo ezahlukeneyo zesayensi kunye nezoshishino.
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I-Alpha-D-Glucose pentaacetate CAS: 3891-59-6
I-Alpha-D-glucose pentaacetate yi-chemical compound efunyenwe nge-acetylating amaqela e-hydroxyl ye-alpha-D-glucose kunye namaqela amahlanu e-acetyl.Isetyenziswa ngokuqhelekileyo kwi-organic synthesis njengeqela elikhuselayo kumaqela e-hydroxyl akhoyo kwi-carbohydrates.Isenokusetyenziswa njengekhompawundi yereferensi kuphando lweekhemikhali kunye nohlalutyo, kwaye njengesixhobo sokuqala sokwenziwa kweekhompawundi ezahlukeneyo.Ukongezelela, i-glucose pentaacetate iphandwe ngokusetyenziswa kwayo kwiinkqubo zokuhanjiswa kweziyobisi, ngenxa yeempawu zayo ezilawulwayo zokukhululwa.
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I-AMPD CAS: 115-69-5 Ixabiso lomvelisi
I-2-Amino-2-methyl-1,3-propanediol, eyaziwa ngokuba yi-AMPD okanye i-α-methyl serinol, i-chemical compound kunye ne-molecular formula C4H11NO2.Yi-alcohol ye-amino esetyenziswa ngokuqhelekileyo njengekhemikhali ephakathi kwi-synthesis yamachiza kunye ne-organic compounds.I-AMPD iyaziwa ngokukwazi ukusebenza njenge-chiral encedisayo kwiimpendulo ze-asymmetric, okwenza ukuba ixabiseke kwimveliso ye-enantiomerically pure compounds.Ukongeza, isetyenziswe njengesithako kukhathalelo lomntu kunye neemveliso zokuthambisa kwiipropathi zayo zokuthambisa.
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I-CAPS SODIUM ESITITY CAS:105140-23-6
Ityuwa yesodium yeCAPS sisithinteli se-zwitterionic esiqhele ukusetyenziswa kwi-biochemical and molecular biology applications.Inexabiso le-pKa elimalunga ne-10.4, iyenza isebenze kuluhlu lwe-pH phakathi kwe-9.7 kunye ne-11.1.Ityuwa yesodium yeCAPS isetyenziswa kwiprotein electrophoresis, reactions enzymatic, i-biological and chemical assays, kunye nemidiya yenkcubeko yeseli.Inika ukuxhathisa kwiinguqu ze-pH ezibangelwa ukungcola kwaye ine-solubility efanelekileyo emanzini.
