3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

I-probe ye-fluorescent equkethe iqela le-nitrobenzoxadiazole (NBD), elibonisa iimpawu ezinamandla ze-fluorescence.Iqela le-aldehyde livumela ukubhaliswa okuthe ngqo kunye nokufunyanwa kwee-biomolecules ezifana neeprotheni, i-nucleic acids, kunye ne-lipids.

I-NBD-aldehyde isoloko isetyenziswa njengedayi esebenzayo kwiimvavanyo zokufumanisa kunye nokulinganisa iprotheni okanye i-peptide conjugation reactions, ukuguqulwa kweprotheyini, kunye neprotheni-protein interactions.Inokuthi ilebhile amaqela e-amino asimahla kwiiprotheyini okanye iipeptide, zenze iibhondi ezizinzileyo.Oku kwenza kube luncedo kwizicelo ezifana ne-immunocytochemistry, immunohistochemistry, kunye ne-western blotting.

I-fluorescence ye-NBD-aldehyde inokuvuyiswa nge-ultraviolet okanye ukukhanya okuluhlaza okwesibhakabhaka, kwaye ikhupha i-fluorescence eluhlaza.Le propati yenza imbonakalo kunye nobungakanani bee-biomolecules ezibhalwe zisebenzisa i-fluorescence microscopy okanye ubuchule be-spectroscopy.