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I-Vancomycin hydrochloride CAS:1404-93-9
I-Vancomycin hydrochloride liyeza lokubulala iintsholongwane elisetyenziselwa ukunyanga izifo ezinzulu ezibangelwa ziintlobo ezithile zeebhaktheriya. Likwiqela leziyobisi ze-glycopeptide kwaye lisebenza ngokuthintela ukwenziwa kwe-peptidoglycan, into ebalulekileyo eludongeni lweeseli zebhaktheriya. Lisetyenziswa rhoqo ukunyanga izifo ezifana ne-MRSA, isifo sohudo esinxulumene neClostridium difficile, kunye ne-bacterial endocarditis..
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I-HEPPS CAS:16052-06-5 Ixabiso lomenzi
I-HEPPS, emele i-N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, yiarhente yokubopha ye-zwitterionic esetyenziswa kakhulu kuphando lwebhayoloji kunye ne-biochemical. Yaziwa ngobuchule bayo obuhle bokubopha kunye nozinzo kuluhlu olubanzi lwe-pH, ngakumbi kuluhlu lwe-pH 6.8-8.2. I-HEPPS idla ngokusetyenziswa kwimidiya yokukhulisa iiseli, ukucocwa kweeproteni, ii-enzyme reactions, kunye nezinye izicelo ezifuna indawo ye-pH elawulwa ngokuchanekileyo. Iyanyibilika emanzini kwaye idla ngokusetyenziswa kumanqanaba aqala kwi-10 mM ukuya kwi-100 mM. I-HEPPS ithathwa njengengenabungozi kwiiseli kwaye ayinakuphazamiseka okuphantsi, okwenza ukuba ibe lukhetho oludumileyo kwiiprotokholi ezininzi zovavanyo.
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I-2,3,5-Triphenyl-2H-tetrazolium chloride CAS:298-96-4
I-2,3,5-Triphenyl-2H-tetrazolium chloride, ekwaziwa ngokuba yi-TTC, yikhompawundi esetyenziswa kakhulu njengesalathisi se-redox kwizicelo zebhayoloji kunye neekhemikhali. Ibonakala njengomgubo wekristale otyheli-omhlophe.
I-TTC isetyenziswa rhoqo kwiimvavanyo ezahlukeneyo zebhayoloji kunye novavanyo lokufumanisa ukuba iiseli ziphila kakuhle na kunye nomsebenzi we-metabolism. Xa yongezwa kwiiseli eziphilayo, incitshiswa nge-enzyme ukuze yenze imveliso ebomvu ye-formazan. Olu nciphiso luxhomekeke kwii-enzymes ezisebenzayo ze-dehydrogenase ezikhoyo kwiiseli ezisebenzayo. Ngenxa yoko, ubungakanani be-formazan ebomvu eveliswayo bulingana nenani leeseli ezisebenzayo kwi-metabolism.
Ubuchule be-TTC bokuvavanya ukuphila kweeseli buyenza ibe luncedo kwiindawo zophando ezifana novavanyo lwe-cytotoxicity, uvavanyo lokwanda kweeseli, kunye novavanyo lokusebenza kakuhle kwamayeza. Ukongeza, isetyenziswa kwi-microbiology ukuvavanya ukukhula kweebhaktheriya, kunye nezifundo ezibandakanya inkcubeko yezicubu zezityalo kunye novavanyo lokuphila kwezicubu.
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Ityuwa ye-p-nitroanilide p-toluenesulfonate ye-Gly-Pro CAS:65096-46-0
Ityuwa ye-Gly-Pro p-nitroanilide p-toluenesulfonate yikhemikhali esetyenziswa kwiimvavanyo ze-enzyme ukulinganisa umsebenzi wee-peptidases ezithile ze-proline. Iqulathe i-dipeptide i-Gly-Pro edityaniswe neqela le-p-nitroanilide, enokukhutshwa sisenzo see-peptidases ezithile ze-proline.
Kwiimvavanyo ze-enzyme, le compound yongezwa kwisampulu equlethe i-enzyme enomdla. I-enzyme iqhawula unxibelelwano phakathi kwe-glycine (Gly) kunye ne-proline (Pro), ikhulula iqela le-p-nitroanilide. I-p-nitroanilide ekhutshiweyo inokulinganiswa nge-spectrophotometric, ivumela ukuqinisekiswa komsebenzi we-enzyme.
Uhlobo lwetyuwa ye-p-toluenesulfonate ye-Gly-Pro p-nitroanilide inika uzinzo kunye nokunyibilika kwizisombululo zamanzi, nto leyo eyenza kube lula ukuyisebenzisa kwiimvavanyo ze-enzyme..
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I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8
I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide yikhompawundi esetyenziswa kwizifundo ezahlukeneyo zebhayoloji, ingakumbi ekufumaneni nasekuboniseni umsebenzi we-enzyme. Yi-substrate enokuthi i-hydrolyzed zii-enzyme ezithile, nto leyo ekhokelela ekukhutshweni kwemveliso enemibala okanye ekhanyayo.
Le khompawundi isetyenziswa rhoqo kwiimvavanyo ukuze kufunyanwe ubukho kunye nomsebenzi wee-enzyme ezifana ne-beta-galactosidase kunye ne-beta-glucuronidase. Ezi enzymes ziqhekeza amaqela e-acetyl kunye ne-glucosaminide kwi-substrate, nto leyo ekhokelela ekudalweni kwe-chromophore eluhlaza okwesibhakabhaka okanye eluhlaza.
Ulwakhiwo olukhethekileyo lwe-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide luvumela ukubonwa lula kunye nokulinganiswa komsebenzi we-enzyme. Ukusetyenziswa kwayo kwiindlela ezahlukeneyo zovavanyo, kubandakanya i-histochemistry, i-immunohistochemistry, kunye novavanyo olusekelwe kwiiseli, kuye kwanegalelo ekuqondeni ngcono imisebenzi ye-enzyme.
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I-AMPPD CAS: 122341-56-4 Ixabiso lomenzi
I-AMPPD, mfutshane ngenxa I-3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, sisixhobo se-chemiluminescent esisetyenziswa kakhulu kwizicelo zebhayoloji kunye ne-immunoassay. Isetyenziswa kakhulu kuphando lwebhayoloji yemolekyuli, ukuxilongwa, kunye nesayensi yobomi.
I-AMPPD yenzelwe ngokukodwa ukusetyenziswa kunye ne-alkaline phosphatase enzyme conjugates. Xa ivezwa kwi-alkaline phosphatase, i-AMPPD ifumana impendulo ekhawulezayo nesebenzayo ye-enzyme cleavage, ekhokelela ekukhutshweni kokukhanya.
Ukukhanya okukhutshiweyo okuvela kwi-AMPPD kubonwa kusetyenziswa iinkqubo zomfanekiso ezibuthathaka okanye ii-luminometers, okuvumela ukulinganiswa kunye nokubonwa kweemolekyuli okanye ii-analytes ekujoliswe kuzo. Le ndlela yokufumanisa esekelwe kwi-chemiluminescence inika uvakalelo oluphezulu, ingxolo esezantsi yangasemva, kunye noluhlu olubanzi lwe-dynamics.
Ngenxa yokuzinza kwayo kunye nesibonakaliso esihlala ixesha elide, i-AMPPD ifanelekile kwizicelo ezifana ne-Western blotting, i-ELISA (i-enzyme-linked immunosorbent assay), i-DNA hybridization, kunye ne-in situ hybridization. Ivumela abaphandi ukuba bafumane kwaye bahlalutye iiproteni, ii-nucleic acids, ii-antibodies, kunye nezinye ii-biomolecules.
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Ityuwa yesodium ye-2,6-Dichloroindophenol CAS:620-45-1
I-3,5-Dichlorophenyldiazonium tetrafluoroborate yikhemikhali esetyenziswa kakhulu kwi-organic synthesis. Yityuwa ye-diazonium, oko kuthetha ukuba iqulethe iqela le-diazonium (N2+) elinamathele kwiringi ye-phenyl ethathelwa indawo yi-athomu ze-chlorine kwindawo yesi-3 neyesi-5.
Le khompawundi idla ngokusetyenziswa kwiimpendulo ezibandakanya ukudibana nezinye iikhompawundi ze-aromatic. Ingadlula kwiimpendulo ezifana nokutshintshwa kwe-electrophilic aromatic, apho inokudlulisela iqela layo le-diazonium kwenye ikhompawundi ye-aromatic ye-nucleophilic ukuze yenze ikhonkco elitsha. Oku kuluncedo ngakumbi ekungenisweni kwamaqela asebenzayo kwiindandatho ze-aromatic..
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I-3,3′-Diaminobenzidine CAS:91-95-2
I-3,3′-Diaminobenzidine (DAB) yikhemikhali esetyenziswa kakhulu kwiindlela zokufaka imibala kwi-immunohistochemistry (IHC) nakwi-immunocytochemistry (ICC). Yi-chromogenic substrate evelisa imveliso yempendulo emdaka xa ivezwe kwii-enzymes ze-peroxidase, ebonisa ubukho be-antigen ekujoliswe kuyo.
I-DAB idla ngokusetyenziswa ukuze ibone iiproteni okanye ezinye ii-biomolecules ezinomdla kwiisampuli zebhayoloji, ezifana namacandelo ezicubu okanye iiseli. Ingabonelela ngophawu olubonakalayo nolubonakalayo lwe-antigen ekujoliswe kuyo, okuvumela abaphandi ukuba bachonge kwaye bahlalutye ukusasazwa kwayo kunye nobuninzi bayo.
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(±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid I-CAS:53188-07-1
I-Trolox yikhompawundi eyenziweyo ekwiklasi yee-antioxidants eyaziwa ngokuba yi-vitamin E analogs. Yi-antioxidant enamandla enokuhlutha ii-free radicals kwaye ikhusele iiseli kunye nezicubu kumonakalo we-oxidative. I-Trolox ifundwe ngokubanzi ngeenzuzo zayo zempilo, kubandakanya amandla ayo okunciphisa ukudumba, ukuthintela umonakalo weeseli, kunye nokuxhasa impilo yentliziyo. Isetyenziswa kakhulu njengekhompawundi yokubhekisa kuphando lwe-antioxidant kwaye njengesongezelelo kwiimveliso ezahlukeneyo zokutya kunye nezobuhle.
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I-1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride CAS:25952-53-8
I-1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, ekwaziwa ngokuba yi-EDC hydrochloride okanye i-EDAC, yikhemikhali esetyenziswa kakhulu kwi-biochemistry nakwi-molecular biology njenge-coupling agent yokuvuselela amaqela e-carboxyl. Yi-white crystalline powder enyibilikayo kakhulu emanzini.
I-EDC hydrochloride isetyenziswa kakhulu ekuhlanganiseni iipeptides kunye neeproteni ngokuncedisa ukwakheka kwee-amide bonds phakathi kwamaqela e-carboxyl kunye nee-amino. Isebenza ngokuvuselela amaqela e-carboxyl, asabela kunye namaqela e-amino xa kukho i-agent edibeneyo efana ne-N-hydroxysuccinimide (NHS). Le mpendulo iphumela ekwakhekeni kwee-amide bonds ezizinzileyo.
I-EDC hydrochloride ikwasetyenziswa kuphando lwe-DNA kunye ne-RNA lokuguqula nokudibanisa ii-nucleic acids, kunye nokuthintela ukuhamba kwee-enzymes kunye nezinye ii-biomolecules kwiindawo eziphezulu. Yi-reagent eguquguqukayo enokuphucula ukusebenza kakuhle kunye nokuchaneka kwezi mpendulo.
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I-HEPES CAS: 7365-45-9 Ixabiso lomenzi
I-HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) sisithinteli sebhayoloji esisetyenziswa kakhulu kuphando lwebhayoloji kunye neekhemikhali. Yaziwa ngokukwazi kwayo ukugcina i-pH ezinzileyo kwizisombululo zamanzi, okwenza ifaneleke kwiintlobo ngeentlobo zezicelo ezibandakanya iinkqubo zeseli kunye ne-enzymatic. I-HEPES yikhompawundi ye-zwitterionic, oko kuthetha ukuba ineentlawulo ezilungileyo nezingalunganga kumaqela ahlukeneyo asebenzayo, nto leyo eyivumela ukuba imelane notshintsho kwi-pH olubangelwa kukongezwa kwee-asidi okanye iziseko. Isetyenziswa rhoqo kwinkcubeko yeseli, uvavanyo lwee-enzyme, izifundo zeproteni, iimvavanyo ze-electrophoresis, kunye nefomyula yamayeza.
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I-p-Nitrophenyl -aL-Fucopyranoside CAS:10231-84-2
I-p-Nitrophenyl-α-L-Fucopyranoside yikhemikhali ekwiklasi ye-nitrophenyl glycosides. Isetyenziswa kakhulu kuphando lwe-biochemical njengesiseko se-enzyme α-L-fucosidase, evuselela i-hydrolysis yayo. Le khemikhali ayinambala kwaye iyanyibilika kwi-organic solvents, nto leyo eyenza kube lula ukuyivavanya kwi-enzyme.
