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I-TAPSO CAS: 68399-81-5 Ixabiso lomenzi
I-TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) yi-zwitterionic buffer esetyenziswa kakhulu kuphando lwebhayoloji kunye ne-biochemical. Yi-arhente esebenzayo yokukhusela ene-pKa esondele kwi-pH ye-physiological, okwenza ifaneleke ukugcina i-pH ezinzileyo kwiimvavanyo zebhayoloji. I-TAPSO idla ngokusetyenziswa ekucoceni iiproteni, uvavanyo lwee-enzyme, inkcubeko yeeseli, kunye nokusetyenziswa kwe-electrophoresis. Ukunyibilika kwayo emanzini amaninzi kunye nokuphazamiseka okuncinci kwiinkqubo zebhayoloji kwenza ukuba ibe lukhetho oludumileyo kuluntu lwezenzululwazi. I-TAPSO yaziwa ngempembelelo yayo encinci kumsebenzi wee-enzyme kwaye idla ngokusetyenziswa njengendlela eyahlukileyo kwezinye ii-agent zokukhusela ezifana ne-Tris okanye ii-phosphate buffers.
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I-6-Methyl-2-pyridinyl2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside CAS:149263-94-5
I-6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside, ekwaziwa ngokuba yi-N-acetylglucosamine thiolactone, yikhemikhali equlathe isangqa se-pyridine, iqela le-acetylamino, kunye nenxalenye ye-thiolactone.
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I-Sodium Glycocholate CAS:863-57-0
I-Sodium glycocholate yikhompawundi yetyuwa ye-bile enceda ekugayweni nasekufunxeni amafutha okutya. Yenziwe kwi-glycine kunye ne-cholic acid kwaye idlala indima ebalulekileyo ekuqhekezeni amafutha abe ngamathontsi amancinci, yandisa indawo yawo engaphezulu ukuze kugaywe kakuhle. I-Sodium glycocholate iyanyibilika kakhulu emanzini kwaye isetyenziswa kwimixube yamayeza ukuphucula ukunyibilika kunye nokufumaneka kwamayeza anganyibilikiyo kakuhle. Ukongeza, isetyenziswa kuphando nakwiindawo zelebhu ukuxhasa ukukhula kweebhaktheriya ezithile kunye neentlobo zeeseli..
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IITAPS Ityuwa yeSodium I-CAS: 70331-82-7
Ityuwa yesodium yeTAPS, ekwaziwa ngokuba yiN-(Tris(hydroxymethyl)methyl)-2-aminoethanesulfonic acid sodium salt, sisixhobo esiguquguqukayo sokulawula i-pH. Isetyenziswa kakhulu kuphando lwebhayoloji kunye nolweekhemikhali, amayeza, kunye nokuxilongwa. ITAPS-Na yikhompawundi ye-zwitterionic enceda ukugcina i-pH ezinzileyo kwizisombululo, okwenza ifaneleke kwiimeko ezahlukeneyo zovavanyo. Ukusetyenziswa kwayo kubandakanya inkcubeko yeseli, uphando lweprotheni, i-electrophoresis, ukwenziwa kweekhemikhali, kunye neefomyula zamayeza. ITAPS-Na luhlobo lweTAPS olunyibilikayo noluzinzileyo olulula ukuluphatha nokulusebenzisa kwizisombululo zamanzi.
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I-NBD chloride CAS: 10199-89-0
I-NBD chloride, ekwaziwa ngokuba yi-7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, yidayi ekhanyayo esetyenziswa kakhulu kwiikhemikhali nakwizinto eziphilayo. Ikwiklasi yee-nitrobenzoxadiazole (NBD) derivatives, ezibonisa iimpawu ezinamandla ze-fluorescence.
I-NBD chloride ibonakaliswa kukwazi kwayo ukwenza ii-covalent bonds kunye nee-nucleophiles, ezifana nee-primary amines, ii-thiols, kunye namaqela e-hydroxyl. Le propati ivumela ukuba isetyenziswe njenge-arhente yokubhala iilebula ze-biomolecules, kubandakanya iiproteni, ii-peptides, ii-nucleic acids, kunye nezinye ii-molecules ezincinci.
Xa i-NBD chloride isabela kwi-nucleophile, ifumana i-replacement reaction, nto leyo ebangela ukuba kuvele imveliso ezinzileyo ene-NBD. Le mveliso ine-lebel ibonisa i-fluorescence emission enamandla, nto leyo eyenza ukuba ibe luncedo kwiindlela ezahlukeneyo zokufumanisa kunye nemifanekiso.
Ukukhutshwa kwe-fluorescence kwee-molecules ezineelebheli ze-NBD kunokuvuselelwa kukukhanya kwe-ultraviolet (UV) malunga ne-470 nm, kwaye ukukhanya okukhutshiweyo kudla ngokubonwa malunga ne-530 nm. Olu bude be-emission luwela kummandla oluhlaza we-spectrum ebonakalayo, apho ukuphazamiseka kokufumanisa okuvela kwiisampuli zebhayoloji kuphantsi kakhulu.
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I-Proteinase K CAS:39450-01-6 Ixabiso lomenzi
I-Proteinase K yi-enzyme ye-serine protease esebenza kakhulu enokubangela i-hydrolysis yeebhondi ze-peptide kwiiproteni. Ivela kwi-albhamu ye-fungus i-Engyodontium kwaye yaziwa ngoluhlu lwayo olubanzi lwezinto ezithile ze-substrate. I-Proteinase K inokuqhawula ngempumelelo iibhondi ze-peptide kwicala le-C-terminal ye-hydrophobic, aliphatic, okanye i-aromatic amino acids, okwenza ukuba ibe luncedo kwiindlela ezahlukeneyo zebhayoloji ye-molecular kunye ne-biochemistry..
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I-N,N-Dimethyl-p-phenylenediamine dihydrochloride CAS:536-46-9
I-N,N-Dimethyl-p-phenylenediamine dihydrochloride yikhemikhali esetyenziswa kakhulu kwizicelo ezahlukeneyo zesayensi kunye nezoshishino. Yipowder yekristale emhlophe ukuya kwemhlophe enefomula yemolekyuli ye-C8H14N2·2HCl.
Le khompawundi inoluhlu olubanzi lokusetyenziswa ngenxa yeempawu zayo kunye nokusebenza kwayo. Idla ngokusetyenziswa njenge-analytical reagent yokumisela iiperoxides kunye ne-dye intermediate ekuvelisweni kwee-colorants ezahlukeneyo kunye nee-pigments.
Kwicandelo lekhemistri yohlalutyo, i-N,N-Dimethyl-p-phenylenediamine dihydrochloride isetyenziswa rhoqo njengesalathisi semibala yokumisela iintlobo ze-oxidative, ezifana ne-hydrogen peroxide okanye ii-peroxides kwiisampuli. I-compound itshintsha umbala xa isabela ngezi arhente ze-oxidizing, nto leyo evumela ukuba zilinganiswe.
Ngaphezu koko, le khemikhali inokufumaneka nakwindlela yokwenza iidayi zeenwele, apho isebenza njengomphuhlisi wombala. Amandla ayo okudala imibala eqaqambileyo nehlala ixesha elide ayenza ithandwe kakhulu kwishishini lokuhombisa.
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Ityuwa ye-Creatine phosphate disodium CAS:922-32-7
Ityuwa ye-Creatine phosphate disodium yikhemikhali equlathe i-creatine, i-amino acid derivative yendalo, edibene namaqela e-phosphate kunye nee-sodium ions. Idlala indima ebalulekileyo ekuboneleleni ngamandla okucutheka kwemisipha kwaye isetyenziswa rhoqo njengesongezelelo sokutya ngabadlali kunye nabakhi bomzimba.
Xa isetyenziswa, ityuwa ye-creatine phosphate disodium iguqulwa ibe yi-creatine phosphate emzimbeni, ize igcinwe kwimisipha. Ngexesha lemisebenzi enamandla kakhulu, efana nokuphakamisa iintsimbi okanye ukubaleka ngesantya esiphezulu, i-creatine phosphate isebenza njengomthombo ofumaneka lula wezibopho ze-phosphate ezinamandla aphezulu, okuvumela ukuphinda kuzaliswe ngokukhawuleza kwe-adenosine triphosphate (ATP).
I-ATP yeyona molekyuli yamandla iphambili esetyenziswa zimisipha, kwaye amanqanaba ayo ancipha ngokukhawuleza ngexesha lokuzilolonga okunamandla. Ngokudibanisa ne-creatine phosphate, abadlali banokwandisa ukufumaneka kwe-ATP, nto leyo ekhokelela ekuphuculeni ukusebenza kwemisipha, ukwanda kwamandla, kunye nokuncipha kokudinwa.
Ukongeza, ityuwa ye-creatine phosphate disodium inokuba nezinye iingenelo ezinokubakho, kubandakanya ukwanda kwemisipha, ukuphucula ukuchacha, kunye nokusebenza kwengqondo okuphuculweyo. Idla ngokuthathwa ngomlomo njengomgubo okanye ithebhulethi, nokuba sisongezelelo esizimeleyo okanye njengenxalenye yerejimeni yokongeza ngaphambi kokuzilolonga okanye emva kokuzilolonga.
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I-2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridiniuM-9-carboxylate 4′-NHS Ester CAS:194357-64-7
I-2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate I-4′-NHS ester yikhemikhali enesakhiwo semolekyuli esintsonkothileyo. Iqulethe iqela le-sulfopropylacridinium kunye neqela elisebenzayo le-carboxylate ester. Ubukho be-ester moiety bubonisa ukuba iyasabela kwaye ingasetyenziswa njenge-arhente yokulebhelisha okanye yokuguqula ii-biomolecules.
Iqela le-sulfopropylacridinium yekhompawundi licebisa ukuba inokuba nokusetyenziswa okunokwenzeka kwiimvavanyo ezisekelwe kwi-fluorescence, apho ingasetyenziswa njenge-fluorescent probe okanye idayi yokufumanisa nokuhlalutya ii-biomolecules. Isenokuba nokubaluleka kwizifundo zeenkqubo zeseli, ezifana ne-intracellular calcium signaling.
Ukufakwa kweqela le-NHS ester kubonisa ukuba inokusabela kunye nee-amines eziphambili, ezifana nezo zifumaneka kwiiproteni okanye iipeptides, ukuze zenze ii-amide bonds ezizinzileyo. Olu hlobo lokusabela lwenza ukuba lube luncedo kwizicelo ze-bioconjugation, apho lunokusetyenziselwa ukulebhelisha okanye ukuguqula ii-biomolecules kunye nezinye iimolekyuli ezisebenzayo, ezifana nee-fluorophores okanye iithegi.
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I-ABEI CAS: 66612-29-1 Ixabiso lomvelisi
I-N-(4-Aminobutyl)-N-ethylisoluminol, ekwaziwa ngokuba yi-ABEI, yikhompawundi ekhanyayo edla ngokusetyenziswa njengeprobe ye-chemiluminescent kwizicelo ze-bioanalytical. Ikwiklasi ye-isoluminol derivatives kwaye isetyenziswa kakhulu kwii-immunoassays kunye neendlela zokufumanisa i-DNA/RNA.
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I-Z-GLY-PRO-PNA CAS: 65022-15-3
I-Z-GLY-PRO-PNA yi-peptide nucleic acid (PNA) derivative ene-sequence Z-Gly-Pro. Le compound isetyenziswa kakhulu kuphando kunye nokuxilongwa ngenxa yokukwazi kwayo ukubopha ngokukodwa kwi-nucleic acid sequences ezongezelelweyo. Ukongezwa kwe-Z-group kwi-N-terminus kuvumela ukujolisa ngokufanelekileyo kunye nokuhanjiswa kwiithagethi zeemolekyuli ezifunwayo. Ngomdibaniso wayo owahlukileyo weempawu ze-peptide kunye ne-nucleic acid, i-Z-GLY-PRO-PNA inika amandla amakhulu kwizicelo ezahlukeneyo, kubandakanya unyango lwe-gene, ukuxilongwa kweemolekyuli, kunye nophuhliso lwamayeza..
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I-TRIS-Acetate CAS: 6850-28-8 Ixabiso lomenzi
I-TRIS-Acetate, sisithambisi esisetyenziswa kakhulu kwiimvavanyo zebhayoloji kunye nezebhayoloji. Yindibaniselwano yesiseko seTris kunye ne-acetic acid, nto leyo ekhokelela kwisisombululo esizinzileyo se-pH esisetyenziselwa ukulawula nokugcina uluhlu lwe-pH olufunekayo kwiintlobo ngeentlobo zezicelo. I-TRIS-Acetate iluncedo kakhulu kwizifundo ze-DNA kunye ne-RNA, njengoko ibonelela ngendawo efanelekileyo yemisebenzi ye-enzyme, i-electrophoresis, kunye ne-gel electrophoresis. Inceda ukugcina uzinzo kunye nokuthembeka kwee-nucleic acids ngexesha leenkqubo ezahlukeneyo, ezinje ngokulandelelanisa i-DNA, i-polymerase chain reaction (PCR), kunye ne-agarose gel electrophoresis. Ukongeza kuphando lwe-nucleic acid, i-TRIS-Acetate ikwasetyenziswa kwiinkqubo zokwahlulwa kweproteni kunye nokucoca, ukukhupha i-membrane protein, kunye novavanyo lwenkcubeko yeseli. Amandla ayo okuguqula ajikeleze ayenza ibe sisixhobo esibalulekileyo kwiindawo ezininzi zophando lwesayensi, ukuqinisekisa iimeko ezifanelekileyo zempendulo yebhayoloji kunye nokugcina uzinzo lwee-enzymes kunye neeproteni.
