I-2'- (4-Methylumbelliferyl) -alpha-DN-acetylneuraminic ityuwa ye-sodium ityuwa yikhemikhali esetyenziswa ngokuqhelekileyo kwiimvavanyo zokuxilonga kunye nophando.Yinto ephuma kwi-fluorescent ebhalwe nge-sialic acid, udidi lwemolekyuli yecarbohydrate efumaneka kumphezulu weeseli.
Le khompawundi isetyenziswa njenge-substrate yee-enzymes ezibizwa ngokuba yi-neuraminidases, esebenza ukususa iintsalela ze-asilic acid kwi-glycoproteins kunye ne-glycolipids.Xa ezi enzyme zisebenza kwi-2'- (4-Methylumbelliferyl) -alpha-DN-acetylneuraminic acid ityuwa yesodium, ikhupha imveliso ye-fluorescent eyaziwa ngokuba yi-4-methylumbelliferon.
I-fluorescence eyenziwa yi-compound inokulinganiswa kunye nokulinganisa, inika ulwazi malunga nomsebenzi we-neuraminidase enzymes.Oku kuluncedo ngakumbi kwisifundo sezifo ezahlukeneyo kunye neemeko ezinxulumene ne-aberrant sialic acid metabolism.
Ikhompawundi ikwasetyenziselwa iinjongo zokuxilonga, ezifana nokufumanisa usulelo lwentsholongwane olubandakanya umsebenzi we-neuraminidase.Kolu vavanyo, ikhompawundi isetyenziselwa ukuchonga ubukho beentlobo ezithile zentsholongwane okanye ukuvavanya ukusebenza kwe-neuraminidase inhibitors kunyango lwe-antiviral.