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I-Dodecyl trimethyl ammonium bromide CAS:1119-94-4
I-Dodecyl trimethyl ammonium bromide, ekwaziwa ngokuba yi-DTAB okanye i-DTB, yi-quaternary ammonium compound esetyenziswa kakhulu njenge-surfactant okanye isepha. Yi-powder emhlophe, eqinileyo enyibilikayo emanzini.
I-DTAB idla ngokusetyenziswa kumashishini ahlukeneyo kunye nezicelo, kubandakanya iimveliso zokhathalelo lomntu, amayeza, kunye neelebhu zophando. Ingasebenza njengearhente yokucoca, i-emulsifier, iarhente yokufutha, okanye iarhente yokumanzisa.
Kwiimveliso zokunyamekela umntu, i-DTAB inokufumaneka kwiishampu, kwii-conditioner, kwiisepha, nakwiindawo zokuhlamba umzimba. Inceda ekudaleni igwebu, yonyuse uzinzo lwemveliso, kwaye iphucule ukusasazeka kwefomyula.
Kwimithi, i-DTAB isetyenziswa ekwenzeni iinkqubo ezahlukeneyo zokuhambisa amayeza, kuquka ii-microemulsions, ii-nanoemulsions, kunye nee-liposomes. Inceda ekunyibilikeni kwamayeza kunye nokuzinzisa, iphucula ukufunxwa kwamayeza kunye nokusebenza kwawo.
Kwiilabhoratri zophando, i-DTAB isetyenziswa rhoqo kwizifundo zebhayoloji nezebhayoloji. Ingasetyenziselwa ukukhupha nokucoca iiproteni ze-membrane, ukukhupha i-DNA, kunye neenkqubo zokuhambisa iijini.
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I-PMSF CAS:329-98-6 Ixabiso lomenzi
I-Phenylmethylsulfonyl fluoride, ekwaziwa ngokuba yi-PMSF, yikhemikhali esetyenziswa kakhulu njenge-serine protease inhibitor. Yi-reactive and powerful reversible inhibitor esebenza ngokubopha nokuguqula indawo esebenzayo ye-serine proteases, ithintela ngempumelelo umsebenzi wazo we-enzyme. I-PMSF iluncedo kakhulu kuphando lwe-biochemical kunye ne-molecular biology ekufundeni ukucocwa kweproteni, ukusebenzisana kweproteni kunye neproteolysis. Ihlala isetyenziselwa ukukhusela iiproteni ekuwohlokeni ngexesha leenkqubo zokucoca kunye nokuthintela umsebenzi weprotease kwi-lysates zeseli kunye ne-extracts.
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I-Ethylenediaminetetraacetic acid i-dipotassium salt dihydrate CAS:25102-12-9
I-Ethylenediaminetetraacetic acid i-dipotassium salt dihydrate, ekwaziwa ngokuba yi-EDTA Dipotassium, yikhemikhali equlathe ifomula yemolekyuli i-K2(C10H14N2O8)·2H2O. Yipowder ekristale esetyenziswa rhoqo njenge-arhente ye-chelating, oko kuthetha ukuba inokubopha kwi-ion zesinyithi. I-EDTA Dipotassium yaziwa ngokukwazi kwayo ukususa okanye ukususa ii-ion zesinyithi kwizisombululo, nto leyo eyenza ukuba ibe luncedo kwiinkqubo ezahlukeneyo zoshishino, ezinje ngonyango lwamanzi, ukulungiswa kokutya neziselo, kunye nefomyula zamayeza. Ikwasetyenziswa kwikhemistri yohlalutyo kunye nonyango lwezonyango ngenxa yeempawu zayo ze-chelation zesinyithi.
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I-ethylenediaminetetraacetic acid tripotassium salt dihydrate CAS:65501-24-8
I-ethylenediaminetetraacetic acid i-tripotassium salt dihydrate, eyaziwa ngokuba yi-EDTA Tripotassium, yikhompawundi equlethe ii-ion ezintathu ze-potassium (K+) kwi-molecule nganye, kunye nee-molecule ezimbini zamanzi (H2O). I-EDTA Tripotassium sisiphumo se-ethylenediaminetetraacetic acid (EDTA) kwaye sineempawu ezifanayo kunye nokusetyenziswa okufanayo.
I-EDTA Tripotassium isetyenziswa kakhulu njenge-chelating agent, oko kuthetha ukuba inamandla okubopha kwi-ion zesinyithi kwaye yenze ii-complexes ezizinzileyo. Ihlala isetyenziselwa ukususa ii-ion zesinyithi ezingafunekiyo kwizisombululo, ngakumbi i-calcium, i-magnesium, kunye nezinye ii-ion zesinyithi esinzima. Ngokuzihlutha ezi i-ion, i-EDTA Tripotassium inokuthintela ukuphazamiseka kwazo okanye iziphumo eziyingozi kwiinkqubo ezahlukeneyo zeekhemikhali kunye neempendulo.
Ukongeza kwiipropati zayo zokuchela, i-EDTA Tripotassium ikwasetyenziswa nakwiindawo ezahlukeneyo zoshishino. Isetyenziswa kakhulu ekucoceni amanzi ukususa ii-ion zesinyithi ezinzima kwimithombo yamanzi, ukuqinisekisa ukhuseleko lwamanzi kunye nomgangatho. Ingafumaneka nakwishishini lokutya neziselo njengesiqinisi kunye nesigcini, ithintela i-oxidation kwaye igcina umgangatho wokutya okucutshungulwayo. Ngaphezu koko, i-EDTA Tripotassium idlala indima kwimixube yamayeza, ikhemistri yohlalutyo, kwaye njengesixhobo esiqhelekileyo sokubhekisa.
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I-Fluorescein Isothiocyante CAS:3326-32-7
I-Fluorescein isothiocyanate (FITC) yidayi ekhanyayo esetyenziswa kakhulu kuphando lwezonyango kunye nokusetyenziswa kokuxilonga. Yinto evela kwi-fluorescein eguqulweyo ukuze iqulathe iqela le-isothiocyanate, eliyivumela ukuba ibopheke ngokudibeneyo kwiiproteni, ii-antibodies, kunye nezinye ii-biomolecules.
I-FITC ikhupha ukukhanya okuluhlaza okuqaqambileyo xa ivuselelwa kukukhanya kobude obufanelekileyo, ngokuqhelekileyo malunga ne-488 nm. Olu kukhanya kunokubonwa kwaye kulinganiswe kusetyenziswa iindlela ezahlukeneyo zokuthatha imifanekiso, ezifana ne-fluorescence microscopy, i-flow cytometry, kunye ne-fluorescence spectroscopy.
Ngokudibanisa i-FITC nee-biomolecules ezinomdla, abaphandi banokulandela baze babone ukusasazwa, indawo, kunye nokusebenzisana kwezi biomolecules ngaphakathi kweeseli, izicubu, okanye iisampuli zebhayoloji. Umzekelo, ii-antibodies ezibhalwe yi-FITC zingasetyenziselwa ukubona iiproteni ezithile okanye ii-antigens kwiimvavanyo ze-immunofluorescence okanye ukuhlalutya iiseli kwiimvavanyo ze-flow cytometry.
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I-GSH CAS: 70-18-8 Ixabiso lomenzi
I-GSH, okanye i-glutathione, yimolekyuli yendalo ye-antioxidant efumaneka emzimbeni. Idlala indima ebalulekileyo ekugcineni ukusebenza kakuhle kweeseli kwaye ibandakanyeka kwiinkqubo ezininzi ezibalulekileyo ze-physiological. I-GSH inceda ukukhusela iiseli ekonakaleni kwe-oxidative ngokunciphisa ii-free radicals eziyingozi kunye neentlobo ze-oxygen ezisebenzayo. Ikwanceda kwiinkqubo zokukhupha ubuthi ngokubopha nokususa iityhefu kunye neesinyithi ezinzima emzimbeni.
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I-BCA-2Na CAS:979-88-4 Ixabiso lomenzi
I-BCA-2Na, ekwaziwa ngokuba yi-Bacillus cereus adenosine triphosphate (ATP) bioluminescent assay, luvavanyo lwe-biochemical olusetyenziselwa ukubona ubukho be-ATP kwisampulu. I-ATP yimolekyuli efumaneka kuzo zonke izinto eziphilayo kwaye isetyenziswa rhoqo njengophawu lokungcola okanye ukucoceka kweentsholongwane. Uvavanyo lwe-BCA-2Na lusebenzisa i-enzyme luciferase ukuququzelela impendulo evelisa ukukhanya xa kukho i-ATP. Ubungakanani bokukhanya okukhutshwayo buyalinganiswa kwaye bunokubonelela ngovavanyo olukhawulezileyo nolunobuzaza lomsebenzi weentsholongwane okanye ukucoceka kumashishini ahlukeneyo, njengokuvelisa ukutya neziselo, ukwenziwa kwamayeza, kunye novavanyo lomgangatho wamanzi. Uvavanyo lwe-BCA-2Na luhlala lusetyenziswa njengendlela ekhawulezayo nethembekileyo yokujonga ucoceko kunye nokulawula umgangatho.
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I-GSSG CAS:27025-41-8 Ixabiso lomenzi
I-GSSG, eyaziwa ngokuba yi-oxidized glutathione, luhlobo lwe-disulfide lwe-antioxidant glutathione (GSH). Ngelixa i-GSH iluhlobo olunciphileyo nolusebenzayo, i-GSSG iluhlobo lwe-oxidized nelungasebenziyo. I-GSSG yenziwa xa i-GSH inikela ngee-electron zayo ukuze inciphise ii-free radicals okanye ezinye iintlobo ezisabelayo. Olu tshintsho lunceda ekukhuseleni umzimba kumonakalo we-oxidative. I-GSSG inokuguqulwa ibuyele kwi-GSH ngenkqubo yokuphinda isetyenziswe equka i-enzyme glutathione reductase. Ukubeka esweni umlinganiselo we-GSH kwi-GSSG emzimbeni kuluncedo ekuvavanyeni imeko ye-antioxidant kunye nempilo iyonke.
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I-BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1
Ityuwa ye-5-Bromo-4-chloro-3-indolyl phosphate disodium yikhompawundi eyenziweyo esetyenziswa kakhulu kuphando lwe-biochemical njengesiseko se-chromogenic sokufumanisa umsebenzi wee-enzymes ze-alkaline phosphatase. Idla ngokubizwa ngokuba yi-BCIP ngamafutshane.
Xa i-alkaline phosphatase isebenza kwi-BCIP, iyanqumla iqela le-phosphate, nto leyo ebangela ukwakheka kwe-blue-purple precipitate. Olu tshintsho lombala luvumela abaphandi ukuba babone kwaye balinganise ubukho be-alkaline phosphatase kwisampuli.
I-BCIP isetyenziswa rhoqo kwiindlela ezahlukeneyo zokusetyenziswa, kubandakanya i-immunohistochemistry, iindlela ze-protein kunye ne-nucleic acid blotting, kunye nee-enzyme-linked immunosorbent assays (ELISAs).
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Iodoacetamide CAS:144-48-9 Ixabiso lomenzi
Iodoacetamide yikhemikhali esetyenziswa kakhulu kuphando lwe-biochemistry kunye ne-molecular biology. Yi-thiol-reactive compound eguqula ngokukodwa ii-cysteine residues kwiiproteni ngokwenza i-covalent bond. Olu tshintsho ludla ngokusetyenziswa kwiindlela zohlalutyo lweproteni ukuvimba ii-cysteine residues, njengakwizifundo zeproteomics apho isetyenziselwa ukulebhelisha ngokukhetha kunye nokuvimba ii-cysteine residues ngaphambi kokugaya iiproteni kunye nohlalutyo lwe-mass spectrometry. Iodoacetamide ikwasetyenziswa kwizifundo zesakhiwo seproteni kunye nokuhlanganiswa kwee-peptides ezineelebheli..
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Ityuwa yeBCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine CAS:6578-06-9
Ityuwa ye-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine yikhemikhali esetyenziswa kakhulu kwi-molecular biology kunye nophando lwe-biochemistry. Ihlala isetyenziswa kwi-enzyme-linked immunosorbent assays (ELISA) kunye neendlela zokubhala iilebhile zeproteni.
Le khompawundi yi-substrate ye-alkaline phosphatase, i-enzyme esetyenziswa kakhulu kwi-molecular biology ukuze kufunyanwe ubukho bee-molecules okanye iiproteni ezithile ngokusebenzisa i-colorimetric okanye i-chemiluminescent reactions.
Xa i-enzyme ye-alkaline phosphatase ikhona, iqhekeza amaqela e-phosphate kwityuwa ye-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, nto leyo ebangela ukwakheka kwedayi eluhlaza okwesibhakabhaka okanye emfusa. Olu tshintsho lombala lunokubonwa kwaye lulinganiswe lula, nto leyo evumela ukulinganiswa komsebenzi we-enzyme okanye ubukho beemolekyuli ekujoliswe kuzo.
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I-N,N-Dimethyl-1,4-phenylenediamine dihydrochloride CAS:536-46-9
I-N,N-Dimethyl-1,4-phenylenediamine dihydrochloride yikhemikhali ebizwa ngokuba yi-DMPD dihydrochloride. Yinto eqinileyo, ekristale enyibilikayo emanzini nakwezinye izinyibilikisi ze-polar.
I-DMPD dihydrochloride idla ngokusetyenziswa kwiindlela ezahlukeneyo zokusabela kweekhemikhali kunye neenkqubo, kubandakanya i-oxidation kunye ne-reduction reactions. Ingasebenza njengomthombo wee-electron okanye njenge-electron acceptor, nto leyo eyenza ukuba ibe luncedo ekuhlanganiseni iidayi, amayeza, kunye nezinye izinto eziphilayo.
Ukongeza kwi-reactivity yayo, i-DMPD dihydrochloride yaziwa ngeempawu zayo zokunciphisa kakhulu. Ingasetyenziswa njenge-reductant kwiindlela ze-analytical chemistry, ezifana ne-spectrophotometry, apho inceda khona ukulinganisa uxinzelelo lwezinto ezithile.
