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I-Tapso Sodium CAS: 105140-25-8 Ixabiso lomenzi
i-3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid,Into mnaI-arhente yokubhafa ye-zwitterionic ngendlela yetyuwa yesodium. Isetyenziswa rhoqo kwiimvavanyo zebhayoloji ye-biochemical kunye neye-molecular ukugcina i-pH ezinzileyo kwizisombululo.It Iyanyibilika kakhulu emanzini kwaye ine-pKa esondele kwi-pH ye-physiological, nto leyo eyenza ukuba ifaneleke kwiintlobo ngeentlobo zezicelo ezifana nokucocwa kweproteni, uvavanyo lwe-enzyme, inkcubeko yeseli, kunye ne-electrophoresis. Ulwakhiwo lwayo olukhethekileyo lweekhemikhali kunye nokuhambelana kwayo neenkqubo zebhayoloji kuyenza ibe yinxalenye ebalulekileyo kwiiprotokholi ezahlukeneyo zelebhu.
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Ityuwa ye-4-Nitrophenyl phosphate di(tris) CAS:68189-42-4
Ityuwa ye-4-Nitrophenyl phosphate di(tris) yikhemikhali esetyenziswa kuphando lwebhayoloji kunye nokuxilongwa. Isetyenziswa kakhulu njengesiseko sovavanyo lwe-enzyme ye-alkaline phosphatase. Le tyuwa, xa i-hydrolyzed yi-alkaline phosphatase, ivelisa imveliso enombala otyheli enokulinganiswa ngokulula nge-spectrophotometric. Le mpendulo isetyenziswa kakhulu kwizicelo ezahlukeneyo, kubandakanya uvavanyo lwe-enzyme-linked immunosorbent assays (ELISAs) kunye nokufunyanwa komsebenzi we-phosphatase. Ubungakanani bombala otyheli buhambelana ngqo nomsebenzi we-enzyme, okuvumela ukulinganiswa ngokuchanekileyo kwamanqanaba e-alkaline phosphatase kwiisampuli zebhayoloji. Ityuwa ye-4-Nitrophenyl phosphate di(tris) izinzile, iyanyibilika emanzini, kwaye inobuthathaka kakhulu, okwenza ukuba ibe lukhetho oludumileyo lovavanyo lwe-enzyme kuphando lwebhayoloji nolwezonyango.
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I-Nitrotetrazolium blue chloride CAS:298-83-9
I-Nitrotetrazolium blue chloride, ekwaziwa ngokuba yi-NBT okanye i-NBT-Cl, yikhemikhali esetyenziswa kakhulu kwiimvavanyo zebhayoloji kunye nezebhayoloji. Yipowder etyheli etyheli enyibilikayo emanzini.
I-NBT isetyenziswa rhoqo njengesalathisi sokufumanisa ubukho bee-superoxide radicals okanye ii-enzymes ezizivelisayo. Itshintsha umbala xa incitshiswa zii-superoxide radicals, itshintsha ukusuka kumthubi ukuya kwi-deep blue formazan precipitate. Le mpendulo idla ngokusetyenziswa kwiimvavanyo zokufunda uxinzelelo lwe-oxidative, ukuphefumla kweseli, kunye nomsebenzi wee-enzyme.
I-NBT isetyenziswa kakhulu kwiindlela ezahlukeneyo, ezinje ngokunciphisa i-nitroblue tetrazolium, ukuze kubonwe ukuveliswa kwe-superoxide kwiiseli kunye nezicubu. Ikwasetyenziswe kwi-immunohistochemistry kunye ne-histochemistry ukufumanisa nokuqonda ezinye ii-enzymes kunye nezinto zeseli.
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I-NSP-AS CAS:211106-69-3 Ixabiso lomenzi
Ityuwa yangaphakathi ye-3-[9-((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate yimolekyuli entsonkothileyo equlathe i-acridinium moiety enamathele kwityuwa yangaphakathi ye-propanesulfonate. Iqulethe iqela le-carboxypropyl, iqela le-4-Methxylphenylsulfonyl, kunye neqela le-amine. Le khemikhali isenokuba yenzelwe ukusetyenziswa kwiindawo ezithile ezifana nekhemistri, amayeza, okanye uphando.
Ngaphezu koko, i-NAC ibonise ithemba ekuxhaseni impilo yesibindi ngokunceda ekususeni izinto ezinobuthi, ezifana ne-acetaminophen, isithomalalisi sentlungu esiqhelekileyo. Inokuba nefuthe lokukhusela ekonakaleni kwesibindi okubangelwa kukusela utywala.
Ukongeza kwiipropati zayo zokuxhasa i-antioxidant kunye nenkxaso yokuphefumla, i-NAC iye yaphononongwa ngeenzuzo zayo ezinokubakho kwimpilo yengqondo. Olunye uphando lubonisa ukuba inokuba nefuthe elihle kwiingxaki zemood, ezifana nokudakumba kunye ne-obsessive-compulsive disorder (OCD).
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L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3
I-L-Leucine p-nitroanilide hydrochloride yikhemikhali ekwiqela le-p-nitroanilide derivatives. Yipowder emhlophe ukuya kwemhlophe ekristale esetyenziswa rhoqo kuphando lwe-biochemical kunye novavanyo lwee-enzyme.
I-L-Leucine p-nitroanilide hydrochloride idla ngokusetyenziswa njengesiseko sokulinganisa umsebenzi wee-enzymes ezifana neeproteases kunye neepeptidases. Xa ezi enzymes ziqhekeza i-L-Leucine p-nitroanilide hydrochloride, ikhupha i-p-nitroaniline, enokufunyanwa nge-spectrophotometric. Izinga lokutshintsha kombala lihambelana ngqo nomsebenzi we-enzyme, okuvumela ukuba kulinganiswe umsebenzi we-enzyme kwiisampuli zebhayoloji.
Le khompawundi iyanyibilika emanzini kwaye inokunyibilika lula kwizisombululo ezifanelekileyo ze-buffer ukuze isetyenziswe kwiimvavanyo ze-enzyme. Kubalulekile ukugcina i-L-Leucine p-nitroanilide hydrochloride kwindawo epholileyo neyomileyo ukuze ihlale izinzile.
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I-Guanidine Thiocyanate Sulfate CAS:593-84-0
I-Guanidine thiocyanate sulfate yikhemikhali ephuma kwi-guanidine, thiocyanate, kunye ne-sulfate ions. Isetyenziswa kakhulu kwi-molecular biology kunye nophando lwe-biochemistry njenge-chaotropic agent.
I-Guanidine thiocyanate sulfate idla ngokusetyenziswa kwiiprotokholi zokwahlulwa kwe-DNA kunye ne-RNA ukuphazamisa ii-membrane zeseli kunye neeproteni ze-denature, okuvumela ukukhutshwa kwee-nucleic acids ngokufanelekileyo. Inceda ekunyibilikiseni i-DNA kunye ne-RNA, ivumela ukwahlulwa kwazo kunye nokucocwa kwiisampuli ezahlukeneyo zebhayoloji.
Ngenxa yeempawu zayo ze-chaotropic, i-guanidine thiocyanate sulfate inceda ekuqhawuleni i-hydrogen bonding kunye nezinye iindlela zokunxibelelana ezingezizo ze-covalent, inceda ekuhlukaniseni ii-nucleic acids kwiiproteni, ii-lipids, kunye nezinye izinto zeseli. Ikwanceda ekuthinteleni umsebenzi we-RNase, iqinisekisa ukuthembeka kunye nobunyulu bee-nucleic acids ezikhutshiweyo..
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Ityuwa yeBCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine CAS:6578-06-9
I-BCIP-Toluidine, ekwaziwa ngokuba yityuwa ye-5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, yikhemikhali esetyenziswa kakhulu kwi-molecular biology kunye nophando lwe-biochemistry. Isetyenziswa kakhulu njengesiseko kwi-enzyme-linked immunosorbent assays (ELISAs) okanye iindlela zokufaka idayi kwi-immunohistochemical ukuze kufunyanwe umsebenzi we-alkaline phosphatase.
Xa i-BCIP-Toluidine iqhekeka yi-alkaline phosphatase, ifumana utshintsho lombala, nto leyo evelisa i-blue-purple precipitate. Le mpendulo ivumela ukubonakala kunye nokulinganiswa komsebenzi we-alkaline phosphatase kwiisampuli. Ubungakanani bombala bunokulinganiswa nge-spectrophotometric kwaye buhambelana ngqo nomsebenzi we-enzyme.
Ukusetyenziswa kwe-BCIP-Toluidine njenge-substrate kuxhaphake kakhulu kwizicelo ezibandakanya ukulebheliswa kwe-DNA okanye i-RNA, njenge-in situ hybridization (ISH) okanye i-Southern and Northern blotting. Ibonelela ngendlela ethembekileyo neyomeleleyo yokufumanisa ubukho bee-nucleic acids ezijoliswe kuzo kwiisampuli.
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I-3-Aminophthalhydrazide CAS:521-31-3
I-3-Aminophthalhydrazide yikhemikhali enefomula yemolekyuli i-C8H8N2O. Ibizwa ngokuba yi-aminohydrazide derivative ye-phthalic acid. Le khompawundi ine-phthalic acid backbone ene-athomu ezimbini ze-hydrogen ezithatyathelwe indawo liqela le-amino kunye neqela le-hydrazine.
I-3-Aminophthalhydrazide inezicelo ezahlukeneyo kwicandelo le-organic chemistry kunye neyeza. Ingasetyenziswa njengesixhobo sokuqala ekuhlanganiseni iikhompawundi ezahlukeneyo zamayeza kunye needayi. Ukongeza, idla ngokusetyenziswa ekulungiseleleni iziseko zeSchiff, ezizizinto eziphambili kwi-organic synthesis.
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I-4-Aminoantipyrine CAS: 83-07-8
I-4-Aminoantipyrine, ekwaziwa ngokuba yi-aminoantipyrine okanye i-aminopyrine, yikhompawundi ye-organic eyenziweyo. Isetyenziswa kakhulu njenge-reagent kwi-analytical chemistry nakwishishini le-pharmaceutical. Ulwakhiwo lwayo lweekhemikhali luqulathe i-aromatic ring ene-amino group (NH2) kunye ne-pyrazolone group, nto leyo eyenza ukuba ibe yikhompawundi eguquguqukayo kwiindlela ezahlukeneyo zokusebenza.
Kwikhemistri yohlalutyo, i-4-Aminoantipyrine isetyenziswa kakhulu njenge-reagent ye-chromogenic kwiimvavanyo ze-colorimetric. Isabela kwizinto ezithile, ezifana nee-phenols kunye nee-peroxides, ukuze zenze ii-complexes ezinemibala ezinokulinganiswa nge-spectrophotometric. Le mpawu iyenza ibe luncedo kwiimvavanyo ezahlukeneyo, kubandakanya nokuchonga ii-enzymes ezifana ne-peroxidase, i-glucose, kunye ne-amylase.
Kwishishini lamayeza, i-4-Aminoantipyrine ibisoloko isetyenziswa njengeyeza lokuthomalalisa iintlungu kunye nelokulwa nokuvuvukala. .
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I-TAPS-NA CAS:91000-53-2 Ixabiso lomenzi
I-N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid ityuwa yesodium, ekwaziwa ngokuba yi-TAPS-Na, yikhemikhali esetyenziswa kakhulu njenge-arhente yokubopha kwizicelo ezahlukeneyo zebhayoloji kunye nezebhayoloji. Yikhompawundi ye-zwitterionic, oko kuthetha ukuba ineentlawulo ezilungileyo nezingalunganga, ezinceda ukugcina i-pH ezinzileyo kwizisombululo.
I-TAPS-Na inoluhlu lwe-pH olumalunga ne-7.7 ukuya kwi-9.1, nto leyo eyenza ukuba ifaneleke ukusetyenziswa okufuna indawo ene-pH ene-alkaline kancinci. Ihlala isetyenziswa kuphando lweprotheni kunye ne-enzyme, kunye neendlela zebhayoloji yemolekyuli ezifana ne-gel electrophoresis kunye ne-DNA sequencing.
Njengetyuwa yesodiyam, iTAPS-Na iyanyibilika kakhulu emanzini, nto leyo evumela ukuba kube lula ukulungiselela izisombululo ezifakwe i-buffer. Yaziwa ngokuba netyhefu encinci kwaye ayinakuphazamisana kangako neemolekyuli zebhayoloji, nto leyo eyenza ukuba ibe lukhetho olukhethwayo kwiimvavanyo ezininzi.
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I-5,5-Dithiobis (i-asidi ye-nitrobenzoic eyi-2) I-CAS:69-78-3
I-5,5-Dithiobis (2-nitrobenzoic acid), ekwaziwa ngokuba yi-DTNB okanye i-Ellman's reagent, yikhemikhali esetyenziswa kakhulu kuphando lwe-biochemical kunye ne-pharmaceutical. Yipowder etyheli, ekristale enyibilikayo kakhulu emanzini nakwi-organic solvents.
I-DTNB isetyenziswa kakhulu ukulinganisa uxinaniso lwamaqela e-sulfhydryl (-SH) kwiiproteni kunye neepeptides. Le compound isabela kunye nee-thiols, nto leyo ebangela ukwenziwa kwe-disulfide exutyiweyo kwaye ikhuphe i-2-nitro-5-thiobenzoate, enombala otyheli onokulinganiswa nge-spectrophotometric. Ngokulinganisa ubunzulu bombala otyheli, kunokwenzeka ukuqikelela uxinaniso lwee-thiols kwisampuli. Le ndlela idla ngokusetyenziswa ukufunda ukusebenzisana kweeproteni neeproteni, ukugoba kweeproteni, kunye neempendulo ze-enzyme ezibandakanya amaqela e-thiol.
Ukongeza kwi-thiol quantification, i-DTNB isetyenzisiwe kwezinye iindlela zokusebenza, ezinje ngokuvavanya imeko ye-redox yeeseli, ukufunda uzinzo lwee-metal-sulfur compounds, kunye nokulinganisa umsebenzi wee-enzymes ezahlukeneyo.
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I-PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) ITYUWA I-CAS:14815-59-9
Ityuwa ye-Phenolphthalein monophosphate i-dicyclohexylammonium yikhemikhali ephuma kwi-phenolphthalein, isalathisi se-pH esisetyenziswa kakhulu kwiindawo zelebhu. Uhlobo lwetyuwa ye-dicyclohexylammonium ye-phenolphthalein monophosphate luhlala lusetyenziswa njenge-analytical reagent kuphando lwe-biochemical kunye ne-molecular biology. Isetyenziswa kakhulu kwiimvavanyo okanye kwiimvavanyo zokufumanisa nokulinganisa ubukho bee-enzyme ezithile, kuquka ezo zibandakanyeka kwi-ATP (adenosine triphosphate) hydrolysis okanye imisebenzi ye-phosphatase. Iimpawu zayo ezikhethekileyo zenza ukuba ibe sisixhobo esiluncedo kuphando lwesayensi olunxulumene ne-enzyme kinetics kunye neenkqubo zeseli..
